Testing method of nucleic acid binding protein based on biochip
A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.
1. A biochip based method for detecting nucleic acid binding proteins, comprising:
1) adding a solution containing several groups of nucleic acid capture probes to a biological sample containing target nucleic acid binding proteins, whereby complexes between the nucleic acid capture probes and the nucleic acid binding proteins are formed, wherein the nucleic acid capture probes contain at least a sequence that target nucleic acid binding proteins can bind, and wherein one strand of the nucleic acid capture probes in each group contains a 3′ overhang;
2) isolating the nucleic acid capture probe-nucleic acid binding protein complexes and collecting the nucleic acid capture probes in the complexes;
3) amplifying the collected nucleic acid capture probes by single-stranded PCR amplification using one primer, wherein the primer can hybridize to the 3′ overhang sequence of the nucleic acid capture probes;
4) hybridizing the amplified capture probes in step 3) with single stranded immobilization probes immobilized on the substrate of a biochip, wherein the immobilization probes contain a sequence complementary to the corresponding nucleic acid capture probes or one strand of the nucleic acid capture probes; and
5) detecting the hybridization result.
2. The method of claim 1 , wherein the isolation of the nucleic acid capture probe-nucleic acid binding protein complexes in step 2) is a process of gel electrophoresis of the mixture sample from step 1), cutting gel slices, and recovering with gel purification kit or electro-elution method to obtain the isolated nucleic acid capture probe-nucleic acid binding protein complexes.
3. The method of claim 1 , wherein the nucleic acid binding proteins are double-stranded DNA (dsDNA) binding proteins, RNA binding proteins, or single-stranded DNA (ssDNA) binding proteins.
4. The method of claim 3 , wherein the dsDNA binding proteins are transcription factors.
5. The method of claim 1 , wherein the nucleic acid capture probes contains a nucleic acid sequence that can be bound by nucleic acid binding proteins.
6. Rewrite as The method of claim 5 , wherein each immobilization probe is complementary to the 3′ overhang sequence of its corresponding nucleic acid capture probe.
7. The method of claim 1 , wherein the 3′ overhang sequence in each group of the nucleic acid capture probes having the 3′ overhang is identical, and the primer sequence is completely complementary to the 3′ overhang sequence.
8. Rewrite as The method of claim 7 , wherein the primer is labeled with a labeling molecule.
9. The method of claim 8 , wherein the 5′ end of the primer is labeled.
10. The method of claim 9 , wherein the labeling molecule is a biotin, a digoxin, a fluorescence dye, a quantum dye, a gold particle, or a nano-particle.
11. The method of claim 1 , wherein the isolation of the nucleic acid capture probe-nucleic acid binding protein complexes in step 2) is chromatographying the mixture sample from step 1) to obtain the isolated nucleic acid capture probe-nucleic acid binding protein complexes.
12. The method of claim 1 , wherein the isolation of the nucleic acid capture probe-nucleic acid binding protein complexes in step 2) is filtering the mixture sample from step 1) with a membrane capable of adsorbing proteins to obtain the isolated nucleic acid capture probe-nucleic acid binding protein complexes.
13. The method of claim 1 , wherein the isolation of the nucleic acid capture probe-nucleic acid binding protein complexes in step 2) is a process of adding antibodies specifically recognizing each nucleic acid binding protein to the mixture sample from step 1) and isolating antibodies to obtain the isolated nucleic acid capture probe-nucleic acid binding protein complexes.
14. The method of claim 1 , wherein the isolation of the nucleic acid capture probe-nucleic acid binding protein complexes in step 2) is a process of separating the mixture sample from step 1) by capillary electrophoresis and collecting the nucleic acid capture probe- nucleic acid binding protein complexes.
15. The method of claim 6 , wherein the overhang of the nucleic acid capture probes is labeled with a labeling molecule.
16. The method of claim 15 , wherein the labeling molecule is a biotin, a digoxin, a fluorescence dye, a quantum dye, a gold particle, or a nano-particle.
17. The method of claim 7 , wherein nucleotides labeled with a labeling molecule are added into the amplification process when using the primer for amplification.
18. The method of claim 17 , wherein the labeling molecule is a biotin, a digoxin, a fluorescence dye, a quantum dye, a gold particle, or a nano-particle.
19. The method of claim 1 , wherein one strand of the capture probes in each group contains a 3′ overhang and a 5′ overhang, and wherein the amplification process is performed with two primers, wherein one primer can hybridize to the 3′ overhang sequence of the nucleic acid capture probes containing the 3′ overhang and the 5′ overhang, and the other primer contains a sequence identical to the 5′ overhang sequence of the nucleic acid capture probes containing the 3′ overhang and the 5′ overhang.
20. The method of claim 19 , wherein the 3′ overhang sequence in each nucleic acid capture probe containing the 3′ overhang and the 5′ overhang is identical; and the 5′ overhang sequence in each nucleic acid capture probe containing the 3′ overhang and the 5′ overhang is identical, wherein one primer can hybridize to the 3′ overhang sequence of the nucleic acid capture probes containing the 3′ overhang and the 5′ overhang, and the other primer contains a sequence identical to the 5′ overhang sequence of the nucleic acid capture probes containing the 3′ overhang and the 5′ overhang.
21. The method of claim 20 , wherein the primers are labeled with a labeling molecule when the two primers are used for amplification.
22. The method of claim 21 , wherein the 5′ end of the primers is labeled.
23. The method of claim 22 , wherein the labeling molecule is a biotin, a digoxin, a fluorescence dye, a quantum dye, a gold particle, or a nano-particle.
24. The method of claim 20 , wherein nucleotides labeled with a labeling molecule are added into the amplification process when using the two primers for amplification.
25. The method of claim 24 , wherein the labeling molecule is a biotin, a digoxin, a fluorescence dye, a quantum dye, a gold particle, or a nano-particle.