COMPOSITIONS AND METHODS FOR TREATMENT OF SKIN DISORDERS
Described herein are compositions and methods of treatment for acne and other skin disorders involving the use of probiotics. In certain aspects, microbes included in the probiotics have been engineered or selected for the treatment of specific skin disorders.
1 . A pharmaceutical composition comprising:
a) a therapeutically effective amount of a health-associated Propionibacterium acnes microbe, wherein the health-associated Propionibacterium acnes microbe is characterized by at least one of the following:
i) comprises at least one gene encoding at least one of a deoxyribose operon repressor and a type II lipase, and less than about 10% pIMPLE plasmid;
ii) comprises at least one gene encoding an ATP binding cassette transporter; or
iii) lacks at least one gene encoding a DNA binding response regulator or a phosphoglycerate kinase, and
b) a pharmaceutically acceptable excipient or biological stabilizer.
2 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe has been engineered or selected to comprise a deoxyribose operon repressor and a type II lipase.
3 . The pharmaceutical probiotic composition of claim 1 , wherein a Cas5 protein is absent from the health-associated Propionibacterium acnes microbe.
4 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe expresses an ATP binding cassette transporter.
5 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe does not express a DNA binding response regulator or a phosphoglycerate kinase.
6 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe comprises an RT1 or RT2 ribotype.
7 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe does not comprise an RT6 ribotype.
8 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe comprises less than about 1% pIMPLE plasmid.
9 . The pharmaceutical probiotic composition of claim 1 , wherein the pharmaceutically acceptable excipient or biological stabilizer increases the viability of the health-associated Propionibacterium acnes microbe at a temperature from about −20° C. to about 30° C.
10 . The pharmaceutical composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe comprises a mixture of two or more different ribotypes of Propionibacterium acnes.
11 . The pharmaceutical probiotic composition of claim 1 , wherein the health-associated Propionibacterium acnes microbe comprises an HP3A11 strain, an HP3B4 strain, an HP4G1 strain, or an HP5G4 strain.
12 . The pharmaceutical probiotic composition of claim 1 , comprising an additional strain of bacteria, wherein the additional strain comprises Propionibacterium avidum, Propionibacterium acnes subsp. defendens , or Propionibacterium granulosum.
13 .- 30 . (canceled)