IP Library › Granted Patent US 12,359,236
Granted Patent B2
US 12,359,236 · App. 17/537,714 · Granted Jul 15, 2025

Production of 2-hydroxyacyl-CoAs and derivatives thereof

Inventors: Ramon Gonzalez (Tampa, FL); Alexander Chou (Houston, TX); James Clomburg (Houston, TX)
Assignee: Mojia Biotech Pte. Ltd.
C12P19/40
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Quick Facts
Patent No.
US 12,359,236
App. No.
17/537,714
Granted
Jul 15, 2025
Kind
B2
Abstract

The production of substituted 2-hydroxyacyl-CoA molecules by a novel reaction is described. The reaction involves the condensation of formyl-CoA with a carbonyl-containing molecule. Such carbonyl-containing molecules include a substituted aldehyde and a ketone. The reaction is catalyzed by enzymes using a TPP-dependent mechanism. Also described is the production of unsubstituted and substituted 2-hydroxyacyl-CoA molecules comprising the condensation of formyl-CoA with a carbonyl-containing molecule, wherein the condensation is catalyzed by a prokaryotic HACL. The 2-hydroxyacyl-CoA can be converted to chemical products having broad applications by using enzyme catalysts. The combination of enzyme catalysts comprises novel biochemical reaction pathways that can be deployed either as polypeptides in a reaction buffer or genetically encoded in recombinant microorganisms.

Claims (44)

1. A method for the production of an alpha-branched 2-hydroxyacyl-CoA comprising condensation of a formyl-CoA with a ketone; wherein the condensation is catalyzed by a TPP-dependent enzyme, wherein the alpha-branched 2-hydroxyacyl CoA is one carbon longer than the ketone, wherein the TPP-dependent enzyme is selected from the group consisting of 2-hydroxyacyl-CoA lyase (HACL), oxalyl-CoA decarboxylase and benzaldehyde lyase.

2. The method of claim 1 , wherein the TPP-dependent enzyme is a 2-hydroxyacyl-CoA lyase (HACL).

3. The method of claim 1 , further comprising producing the formyl-CoA by contacting a one-carbon substrate with an enzyme catalyst, wherein the one-carbon substrate is selected from the group consisting of methane, methanol, formate, formaldehyde and carbon dioxide.

4. The method of claim 1 , further comprising producing the ketone by contacting a substrate with an enzyme catalyst.

5. The method of claim 1 , further comprising converting the alpha-branched 2-hydroxyacyl-CoA to a branched product.

6. The method of claim 3 , wherein the one-carbon substrate is formaldehyde and the enzyme catalyst that produces the formyl-CoA is:

a. an acyl-CoA reductase (acylating aldehyde dehydrogenase) that catalyzes the conversion of the formaldehyde to the formyl-CoA.

7. The method of claim 3 , wherein the one-carbon substrate is methanol and the enzyme catalysts to produce the formyl-CoA are:

a. a methanol dehydrogenase catalyzing the conversion of the methanol to formaldehyde; and

b. an acyl-CoA reductase (acylating aldehyde dehydrogenase) catalyzing the conversion of the formaldehyde to the formyl-CoA.

8. The method of claim 3 , wherein the one-carbon substrate is methane and the enzyme catalysts to produce the formyl-CoA are:

a. a methane monooxygenase catalyzing the conversion of the methane to methanol;

b. a methanol dehydrogenase catalyzing the conversion of the methanol to formaldehyde; and

c. an acyl-CoA reductase (acylating aldehyde dehydrogenase) catalyzing the conversion of the formaldehyde to formyl-CoA.

9. The method of claim 3 , wherein the one-carbon substrate is formate and the enzyme catalysts to produce the formyl-CoA are:

a. an acyl-CoA synthase catalyzing the conversion of the formate to formyl-CoA; or

b. a formate kinase catalyzing the conversion of the formate to formyl-phosphate and a phosphate formyl-transferase catalyzing the conversion of formyl-phosphate to the formyl-CoA.

10. The method of claim 3 , wherein the one-carbon substrate is carbon dioxide and the enzyme catalysts to produce the formyl-CoA are:

a. a carbon dioxide reductase catalyzing the conversion of the carbon dioxide to formate; and

b. an acyl-CoA synthase catalyzing the conversion of the formate to the formyl-CoA; or

c. a formate kinase catalyzing the conversion of formate to formyl-phosphate and a phosphate formyl-transferase catalyzing the conversion of formyl-phosphate to the formyl-CoA.

11. The method of claim 5 , wherein the branched product is a branched aldehyde, wherein the conversion of the alpha-branched 2-hydroxyacyl-CoA to said product is catalyzed by an enzyme catalyst, and wherein the enzyme catalyst is:

a. an acyl-CoA reductase catalyzing the conversion of the alpha-branched 2-hydroxyacyl-CoA to the branched aldehyde.

12. The method of claim 5 , wherein the alpha-branched product is a branched alcohol, wherein the conversion of the alpha-branched 2-hydroxyacyl-CoA to said branched product is catalyzed by enzyme catalysts, and wherein the enzyme catalysts are:

a. an acyl-CoA reductase catalyzing the conversion of the alpha-branched 2-hydroxyacyl-CoA to the branched aldehyde; and

b. an alcohol dehydrogenase (aldehyde reductase) catalyzing the conversion of the branched aldehyde to the branched alcohol.

13. The method of claim 5 , wherein the product is a branched carboxylic acid, wherein the conversion of the alpha-branched 2-hydroxyacyl-CoA to said product is catalyzed by enzyme catalysts, and wherein the enzyme catalysts are:

a. a thioesterase catalyzing the conversion of the branched 2-hydroxyacyl-CoA to the branched carboxylic acid.

14. The method of claim 3 , wherein the enzyme catalysts are contained in a recombinant microorganism harboring the genes for expressing each enzyme.

15. The method of claim 3 , wherein the substrates are contacted with enzyme catalysts by addition of each to an aqueous reaction mixture optionally containing buffers, salts, vitamins, minerals, and cofactors.

16. The method of claim 2 , wherein the 2-hydroxacyl-CoA lyase is a human HACL.

17. The method of claim 16 , wherein the human HACL is Homo sapiens HACL1.

18. The method of claim 2 , wherein the 2-hydroxyacyl-CoA lyase is a prokaryotic HACL.

19. The method of claim 18 , wherein the prokaryotic HACL is from Rhodospiralles bacterium URHD0017.

20. The method of claim 1 , wherein the ketone is a methyl ketone.

21. The method of claim 20 , wherein the methyl ketone has the formula:

wherein R 1 is methyl, and R 2 is an alkyl; and

the alpha-branched 2-hydroxyacyl-CoA has the formula:

22. The method of claim 21 , wherein R 2 is selected from the group consisting of methyl, ethyl and propyl.

23. The method of claim 22 , wherein the ketone is acetone.

24. The method of claim 22 , wherein the ketone is 2-butanone.

25. The method of claim 22 , wherein the ketone is 2-pentanone.

26. The method of claim 1 , wherein the ketone is 3-pentanone and the alpha-branched 2-hydroxyacyl CoA is

27. The method of claim 1 , wherein the ketone is hydroxyacetone and the alpha-branched 2-hydroxyacyl-CoA is 2,3-dihydroxyisobutyryl-CoA.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 11, 2023
From: C1PLUS BIOTECHNOLOGY LLC
To: MOJIA BIOTECH PTE. LTD.
Reel/Frame 064856/0143 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 24, 2023
From: GONZALEZ, RAMON
To: C1PLUS BIOTECHNOLOGY LLC
Reel/Frame 064692/0803 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 24, 2022
From: CHOU, ALEXANDER; CLOMBURG, JAMES
To: RAMON GONZALEZ
Reel/Frame 060883/0398 →
Continuity (3)
Continuation PCTUS2020035827 · Jun 3, 2020
Provisional Application 62856934 · Jun 4, 2019
Related Publication 20220162661A1 · May 26, 2022
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