Method for enzymatic preparation of R-3 aminobutyric acid
An R-3-aminobutyric acid preparation method with high efficiency and high stereoselectivity. The method comprises using aspartase with stereoisomerization catalytic activity derived from Escherichia coli to efficiently convert butenoic acid into R-3-aminobutyric acid. After only 24 h of reaction, the conversion rate is as high as ≥98%, and the ee value is ≥99.9%. The conversion efficiency is greatly improved, the reaction time is shortened, and the production costs are reduced. The method features a high yield, a high conversion rate, low costs, a short production cycle, a simple process, ease of enlargement, suitability for mass production and the like.
1. An R-3-aminobutyric acid production strain expressing a polypeptide, wherein the polypeptide is an exogenous aspartase derived from E. coli and is used to catalyze a stereoisomeric catalytic reaction as shown below:
wherein the exogenous aspartase is a mutant having 90% sequence identity to the amino acid sequence of SEQ ID NO: 5, possessing two to four amino acid mutations in the amino acid sequence corresponding to the wild type aspartase shown in SEQ ID NO: 5; or
wherein the exogenous aspartase is a mutant having an amino acid mutation selected from the group consisting of threonine (T) at position 204, methionine (M) at position 338, Lysine (K) at position 341, asparagine (N) at position 343, and a combination thereof.
2. The R-3-aminobutyric acid production strain of claim 1 , wherein the mutant has one or more mutations selected from the group consisting of T204C, M338I, K341M, N343C, and a combination thereof.
3. The R-3-aminobutyric acid production strain of claim 1 , wherein the exogenous aspartase has the amino acid sequence shown in SEQ ID NO: 3.
4. A polynucleotide encoding a polypeptide, wherein the polypeptide is an exogenous aspartase obtained from E. coli and is used to catalyze a stereoisomeric catalytic reaction as shown below:
wherein the exogenous aspartase is a mutant having 90% sequence identity to the amino acid sequence of SEQ ID NO: 5, which has two to four amino acid mutations in the amino acid sequence corresponding to the wild type aspartase shown in SEQ ID NO: 5; or
wherein the exogenous aspartase is a mutant having an amino acid mutation selected from the group consisting of threonine (T) at position 204, methionine (M) at position 338, Lysine (K) at position 341, asparagine (N) at position 343, and a combination thereof.
5. The polynucleotide of claim 4 , wherein the exogenous aspartase has the amino acid sequence shown in SEQ ID NO: 3.
6. The polynucleotide of claim 4 , wherein the polynucleotide has a sequence as shown in SEQ ID NO: 4.
7. The polynucleotide of claim 4 , wherein the polynucleotide has over 95% homologous to the sequence of SEQ ID NO: 4, and encodes a polypeptide as shown in SEQ ID NO: 3.