Anti-galactofuranose antibodies for detecting and treating aspergillosis
It is provided novel anti-galactofuranose antibodies and their use for diagnosis of and/or treating aspergillosis, and for the design of chimeric antigen receptor T-cells, wherein single chain variable fragment of the antibodies, such as a heavy chain variable region or a light chain variable region, is fused via a spacer and a transmembrane domain to a signaling endodomain to generate an expression cassette that will be integrated into a T cell.
1. An antibody comprising:
a heavy chain amino acid sequence consisting of SEQ ID NO: 2;
and
a light chain amino acid sequence consisting of SEQ ID NO: 4.
2. The antibody of claim 1 , wherein said antibody further comprises a fluorochrome or a labeling molecule.
3. The antibody of claim 2 , wherein said labeling molecule is biotin, peroxidase, alkaline phosphatase, an oligonucleotide labeling, a radiolabel, a metal, glucoamylase or β-galactosidase.
4. The antibody of claim 1 , wherein said antibody binds to a galactofuranose oligosaccharide.
5. The antibody of claim 4 , wherein said antibody detects a polysaccharide comprising a mono-, di-, tri-, or tetrasaccharides of galactofuranose.
6. The antibody of claim 4 , wherein said galactofuranose oligosaccharide is:
7. The antibody of claim 6 , wherein X=0 to 20.
8. The antibody of claim 6 , wherein X=0 to 10.
9. The antibody of claim 6 , wherein X=0 to 3.
10. The antibody of claim 1 , said antibody is attached to a solid support.
11. The antibody of claim 10 , wherein the solid support is an immunostrip.