Spectrofluorophotometer, spectrofluoro-measurement method, and image capturing method
Provided is a spectrofluorophotometer acquiring both a spectrum and a sample image in measurement of fluorescence of a liquid sample. A spectrofluorophotometer includes a light source, an excitation-side spectroscope that performs spectral dispersion of light of the light source and generates excitation light, a fluorescence-side spectroscope that performs spectral dispersion of fluorescence emitted from a sample irradiated with excitation light into monochromatic light, a sample container installation portion for holding a sample container which receives a liquid sample and is formed of a transparent material, a detector that detects fluorescence emitted from the liquid sample, and an image capturing device that captures a sample image of a sample emitting fluorescence. The sample container installation portion includes a port for allowing excitation light to pass therethrough, a port for allowing fluorescence emitted from a sample to pass therethrough, and a port for allowing the image capturing device to observe a sample.
1. A spectrofluorophotometer comprising:
a light source;
an excitation-side spectroscope that performs spectral dispersion from light of the light source and generates excitation light;
a fluorescence-side spectroscope that performs spectral dispersion of fluorescence emitted from a sample irradiated with the excitation light into monochromatic light;
a sample container installation portion for holding a sample container which receives a liquid sample and is formed of a transparent material;
a detector that detects fluorescence emitted from the liquid sample; and
an image capturing device that captures a sample image of the liquid sample emitting fluorescence,
wherein the sample container installation portion has ports in an excitation light incidence direction for transmitting the excitation light to the sample in the excitation light incidence direction and a fluorescence emission direction and further has a port in another direction for the image capturing device to capture the sample image and a port which is provided on a surface facing the port in the excitation light incidence direction and transmits excitation light that has been transmitted through the sample in a linear direction to outside.
2. The spectrofluorophotometer according to claim 1 ,
wherein the image capturing device is provided at a position in a bottom surface direction of the sample container installation portion, and
wherein the image capturing device captures the sample image by directly capturing fluorescence emitted from the sample in the bottom surface direction of the sample container installation portion.
3. The spectrofluorophotometer according to claim 1 ,
wherein the image capturing device is provided at a position in a side surface direction of the sample container installation portion, and
wherein the image capturing device captures the sample image by directly capturing fluorescence emitted from the sample in the side surface direction of the sample container installation portion.
4. A spectrofluoro-measurement method using a spectrofluorophotometer comprising
a light source,
an excitation-side spectroscope that performs spectral dispersion from light of the light source and generates excitation light,
a fluorescence-side spectroscope that performs spectral dispersion of fluorescence emitted from a sample irradiated with the excitation light into monochromatic light,
a sample container installation portion for holding a sample container which receives a liquid sample and is formed of a transparent material,
a detector that detects fluorescence emitted from the liquid sample, and
an image capturing device that captures a sample image of the liquid sample emitting fluorescence,
the spectrofluoro-measurement method comprising:
causing excitation light to be directly incident in an excitation light incidence direction through a port provided in the excitation light incidence direction of the sample container installation portion;
causing excitation light that has been transmitted through the sample in a linear direction to be transmitted through a port provided on a surface facing the port in the excitation light incidence direction to outside;
causing the detector to detect fluorescence through a port provided in a fluorescence emission direction of the sample container installation portion; and
causing the image capturing device to capture the sample image via a port provided in another direction of the sample container installation portion.
5. An image capturing method using a spectrofluorophotometer comprising:
irradiating a sample with excitation light;
blocking excitation light after fluorescence and phosphorescence are emitted from the sample;
detecting only phosphorescence emitted from the sample after excitation light is blocked; and
capturing a sample image in synchronization with a detection timing of phosphorescence.