Instruments and methods for loading cells into implantable devices
Embodiments herein describe tools, instruments and methods for aseptic loading, dispensing and/or delivering cells into an implantable device and aseptically and selectively sealing a device inside a sterile package as well as and storing and preparing for shipment the cell-filled device.
1. A method of loading an implantable device with cells, the method comprising:
a) obtaining a first container comprising a flushing volume, a second container comprising a cell volume, and a third container comprising a priming volume;
b) loading a dosing tube assembly with first the flushing volume from the first container, then the cell volume from the second container, and then the priming volume from the third container; and
c) dispensing first the priming volume, then the cell volume, and then the flushing volume from the dosing tube assembly into the implantable device through a device port to load the implantable device with cells.
2. The method of claim 1 , wherein the flushing volume comprises a liquid substantially without cells, the cell volume comprises the liquid with suspended cells, and the priming volume comprises the liquid substantially without the cells.
3. The method of claim 1 , wherein step (b) comprises aspirating first the flushing volume, then the cell volume, and then the priming volume from the first, second, and third containers, respectively, into the dosing tube assembly under negative pressure generated by a pump.
4. The method of claim 3 , wherein step (c) comprises dispensing first the priming volume, then the cell volume, and then the flushing volume from the dosing tube assembly into the implantable device through the implantable device port under positive pressure generated by the pump.
5. The method of claim 1 , wherein the dosing tube assembly comprises a fluid reservoir tube and a dosing tube.
6. The method of claim 5 , wherein the flushing volume, the cell volume, and the priming volume once aspirated are contained in the fluid reservoir tube.
7. The method of claim 5 , wherein during step (c), the dosing tube is detachably connected to the device port by a fitting.
8. The method of claim 1 , wherein the cells are pancreatic lineage cells or the cells are pancreatic lineage cell aggregates.
9. The method of claim 8 , wherein the pancreatic lineage cells or pancreatic lineage cell aggregates are pancreatic progenitor cells, PDX-1 positive pancreatic progenitor cells, pancreatic endoderm cells, endocrine precursor cells, endocrine cells, immature beta cells, or immature islet cells.
10. The method of claim 1 , wherein the method is automated, semi-automated, or manual.
11. The method of claim 1 , further comprising (d) sealing the device port after step (c).
12. The method of claim 1 , wherein the implantable device comprises multiple device ports, wherein step (b) comprises loading multiple sets of a flushing volume, a cell volume, and a priming volume into the dosing tube assembly from the containers and wherein step (c) comprises dispensing each set of a priming volume, a cell volume, and a flushing volume into the implantable device via one of the device ports.
13. The method of claim 1 , wherein the implantable device comprises multiple implantable devices, wherein step (b) comprises loading multiple sets of a flushing volume, a cell volume, and a priming volume into the dosing tube assembly from the containers and wherein step (c) comprises dispensing each set of a priming volume, a cell volume, and a flushing volume into one of the implantable devices.
14. A method of loading an implantable device with cells, the method comprising:
(a) obtaining a first container comprising a flushing volume, a second container comprising a cell volume, and a third container comprising a priming volume;
(b) aspirating first the flushing volume from the first container, then the cell volume from the second container, and then the priming volume from the third container into a dosing tube assembly under negative pressure generated by a pump, wherein a first end portion of the dosing tube assembly is fluidly coupled to the pump; and
(c) dispensing first the priming volume, then the cell volume, and then the flushing volume from the dosing tube assembly into the implantable device through a device port under positive pressure generated by the pump, wherein during the act of dispensing, a second end portion of the dosing tube assembly is detachably connected to the device port, such that the implantable device becomes loaded with cells.
15. The method of claim 14 , wherein the flushing volume comprises a liquid substantially without cells, the cell volume comprises the liquid with suspended cells, and the priming volume comprises the liquid substantially without the cells.
16. The method of claim 14 , wherein the dosing tube assembly comprises a fluid reservoir tube and a dosing tube, wherein the dosing tube comprises the second end portion of the dosing tube assembly.
17. The method of claim 16 , wherein during step (c), the dosing tube is detachably connected to the device port by a fitting.
18. The method of claim 16 , wherein the dosing tube assembly further comprises a fitting connecting the dosing tube to the fluid reservoir tube.
19. The method of claim 16 , wherein the fluid reservoir tube comprises a portion between the first and second end portions that is wound into a coil.
20. The method of claim 14 , wherein the pump comprises a syringe pump comprising a piston, a cylinder, and a motor configured to control displacement of the piston relative to the cylinder.