Granted Patent B2

US 12,442,037 · App. 17/554,093 · Granted Oct 14, 2025

Inventors: Andreas Geipel (Mettmann, DE); Christian Korfhage (Langenfeld, DE); Frank Reinecke (Essen, DE)

Assignee: Resolve Biosciences GMBH

C12Q1/6841C12Q1/682C12Q1/6876G01N1/30G01N33/56961C12Q2600/16

Abstract

Claims (42)

1. A kit for multiplex analyte encoding, comprising

(A) at least twenty (20) different sets of analyte-specific probes for encoding of at least 20 different analytes, each set of analyte-specific probes binding to a different analyte, wherein the analyte is a nucleic acid and each set of analyte-specific probes comprises at least five (5) analyte-specific probes which specifically bind to different sub-structures of the same analyte by hybridization to form double stranded segments, each analyte-specific probe comprising

(aa) a binding element(S) that specifically binds to one of the different analytes to be encoded by hybridization to form a double stranded segment, and

(bb) an identifier element (T) comprising a specific identifier sequence which identifies the analyte to be encoded, wherein the analyte-specific probes of a particular set of analyte-specific probes share a common identifier element (T), and

wherein the analyte-specific probes of a particular set of analyte-specific probes differ from the analyte-specific probes of another set of analyte-specific probes in the nucleotide sequence of the identifier element (T),

wherein the analyte-specific probes in each set of analyte-specific probes bind to the same analyte and comprise the same nucleotide sequence of the identifier element (T) which is specific to said analyte; and

(B) at least one set of decoding oligonucleotides per analyte, wherein in each set of decoding oligonucleotides for an individual analyte each decoding oligonucleotide comprises:

(aa) an identifier connector element (t) comprising a nucleotide sequence which is essentially complementary to at least a section of the specific identifier sequence of the identifier element (T) of the corresponding analyte-specific probe set such that an identifier connector element (t) and an identifier element (T) form, under annealing conditions, a T/t double stranded segment having a T/t melting temperature, and

(bb) a translator element (c) comprising a nucleotide sequence allowing a specific hybridization of a signal oligonucleotide wherein the translator element (c) is not specific to an analyte;

wherein the decoding oligonucleotides of a set for an individual analyte differ from the decoding oligonucleotides of another set for a different analyte in the identifier connect element (t); and

(C) one set of signal oligonucleotides per signal element, wherein the number of different sets of signal elements is lower than the number of different types of analytes, each signal oligonucleotide comprising:

(aa) a translator connector element (C) comprising a nucleotide sequence which is not specific to an analyte, and which is essentially complementary to at least a section of the nucleotide sequence of a translator element (c) comprised in a decoding oligonucleotide such that a translator element (c) and a translator connector element (C) form, under annealing conditions, a C/c double stranded segment having a C/c melting temperature, and

(bb) a signal element, wherein the signal element for each signal oligonucleotide is a fluorescent label;

wherein the T/t melting temperature and the C/c melting temperature are lower than a temperature necessary to dissociate the S binding element from its analyte such that a T/t double stranded segment and a C/c double stranded segment melt under conditions where the S binding element remains bound to its analyte; wherein the kit further comprises;

(D) at least a set of non-signal connector oligonucleotides for binding to a particular identifier element (T) wherein each non-signal connector oligonucleotide comprises an identifier connector element (t) comprising a nucleotide sequence which is essentially complementary to at least a section of a specific identifier sequence, and does not comprise a translator element (c) comprising a nucleotide sequence allowing a specific hybridization of a signal oligonucleotide.

2. The kit according to claim 1 , wherein the kit comprises at least two different sets of decoding oligonucleotides per analyte,

wherein the decoding oligonucleotides comprised in these different sets comprise the same identifier connector element (t) comprising a nucleotide sequence which is essentially complementary to at least a section of the specific identifier sequence of the identifier element (T) of the corresponding analyte-specific probe set, and

wherein the decoding oligonucleotides of the different sets per analyte differ in the translator element (c) comprising a nucleotide sequence allowing a specific hybridization of a signal oligonucleotide.

3. The kit according to claim 1 , wherein the kit comprises at least two different sets of decoding oligonucleotides per analyte,

wherein the decoding oligonucleotides comprised in these different sets comprise the same identifier connector element (t) comprising a nucleotide sequence which is essentially complementary to at least a section of the specific identifier sequence of the identifier element (T) of the corresponding analyte-specific probe set, and

wherein the decoding oligonucleotides of the different sets for at least one analyte differ in the translator element (c) comprising a nucleotide sequence allowing a specific hybridization of a signal oligonucleotide.

4. The kit according to claim 1 , wherein the number of different sets of decoding oligonucleotides per analyte comprising different translator elements (c) corresponds to the number of different sets of signal oligonucleotides comprising different connector elements (C).

5. The kit according to claim 1 , wherein the decoding oligonucleotides in a particular set of decoding oligonucleotides bind to identical identifier elements (T) which are specific to a particular analyte.

6. The kit according to claim 1 , wherein all sets of decoding oligonucleotides for the different analytes comprise the same type(s) of translator element(s) (c).

7. The kit according to claim 1 , wherein the kit comprises:

(D) at least two (2) different sets of non-signal decoding oligonucleotides for binding to at least two different identifier elements (T) of analyte-specific probes, each set of non-signal decoding oligonucleotides binding to a different identifier element (T),

wherein each non-signal decoding oligonucleotide comprises an identifier connector element (t) comprising a nucleotide sequence which is essentially complementary to at least a section of a specific identifier sequence, and does not comprise a translator element (c) comprising a nucleotide sequence allowing a specific hybridization of a signal oligonucleotide.

8. The kit according to claim 1 , wherein sets of non-signal connector oligonucleotide may be comprised in a pre-mixture of different sets of non-signal connector oligonucleotides or exist separately.

9. The kit according to claim 1 , wherein the kit comprises:

(E) a set of non-signal oligonucleotides, each non-signal oligonucleotide comprising:

(aa) a translator connector element (C) comprising a nucleotide sequence which is essentially complementary to at least a section of the nucleotide sequence of the translator element (c), and

(bb) a quencher (Q), a signal element and a quencher (Q), or does not comprise a signal element.

10. The kit according to claim 1 , wherein the kit comprises:

(E) at least two sets of non-signal oligonucleotides, each non-signal oligonucleotide comprising:

(aa) a translator connector element (C) comprising a nucleotide sequence which is essentially complementary to at least a section of the nucleotide sequence of the translator element (c), and

(bb) a quencher (Q), a signal element and a quencher (Q), or does not comprise a signal element.

11. The kit according to claim 9 , wherein the different sets of non-signal oligonucleotides may be comprised in a pre-mixture of different sets of non-signal oligonucleotides or exist separately.

12. The kit according to claim 1 , wherein the decoding oligonucleotides in a particular set of decoding oligonucleotides binding to identical identifier elements (T) which are specific to a particular analyte.

13. The kit according to claim 1 , wherein the different sets of decoding oligonucleotides may be comprised in a pre-mixture of different sets of decoding oligonucleotides or exist separately.

14. The kit according to claim 1 , wherein the different sets of analyte-specific probes may be comprised in a pre-mixture of different sets of analyte-specific probes or exist separately.

15. The kit according to claim 1 , wherein the different sets of signal oligonucleotides may be comprised in a pre-mixture of different sets of signal oligonucleotides or exist separately.

16. The kit according to claim 1 , wherein the analyte to be encoded is a ribonucleic acid.

Assignments (2)

Continuity (5)

References Cited (67)