OPTIMIZATION OF SCENT OR FLAVOR COMPOSITIONS
This application relates to methods for determining the concentration of an odorant composition for use with an age-restricted product. The methods generally include creating an odor or flavor profile that may be used to either optimize flavors and odors to appeal to certain age groups, or to discourage use by certain age groups. In some instances, odorant compositions may be screened to determine whether they are differentially perceived by age groups.
1 . A method for determining the concentration of an odorant composition for use with a product comprising:
identifying one or more olfactory receptors that bind the odorant composition, wherein the odorant composition is differentially perceived by at least two age groups, and wherein identifying is performed by:
exposing the one or more olfactory receptors to the odorant composition at a plurality of odorant concentrations, the one or more olfactory receptors being coupled to a reporter that produces a signal upon binding of the odorant composition to the one or more olfactory receptors; and
detecting binding of the one or more olfactory receptors to the odorant composition at each odorant concentration by measuring the produced signal;
creating an age-related odor or flavor profile by determining the concentration of the odorant composition resulting in about a 10% to about 90% maximal binding response to the one or more odorant receptors (about EC 10 to about EC 90 concentrations); and
including the odorant composition in the product at the concentration that produces the desired effect in one of the at least two age groups.
2 . The method of claim 1 , wherein the odorant composition at the EC 90 concentration is designated for use in the product by one of the at least two age groups.
3 . The method of claim 1 , wherein the at least two age groups comprise children or adolescents.
4 . The method of claim 1 , wherein the at least two age groups comprises adults.
5 . The method of claim 1 , wherein the odorant composition comprises one or more odorants.
6 . The method of claim 5 , wherein the one or more odorants comprises menthol, mint, banana, onion, or rose.
7 . The method of claim 1 , wherein the odorant composition comprises menthol.
8 . The method of claim 1 , wherein the one or more olfactory receptors is OR51E1, OR8K3, OR2W1, OR2J2, OR11H7P, OR5P3, or OR1G1.
9 . The method of claim 1 , wherein the one or more olfactory receptors is OR52D1 or OR1G1.
10 . The method of claim 1 , wherein the product is an age-restricted product.
11 . The method of claim 10 , wherein the age-restricted product comprises alcohol, cannabis, nicotine, or tobacco.
12 . The method of claim 1 , wherein the one or more olfactory receptors are part of a live cell assay.
13 . The method of claim 1 , wherein the one or more olfactory receptors are provided in a membrane fraction of a host cell.
14 . The method of claim 1 , wherein the one or more olfactory receptors are provided in liposomes.
15 . A method for deterring use of an age-restricted product by children or adolescents comprising:
detecting binding of an odorant composition at a plurality of concentrations by one or more olfactory receptors according to claim 1 , wherein the odorant composition comprises menthol; and
including the odorant composition in the age-restricted product at a concentration ranging from the EC 50 concentration to the EC 90 concentration to deter use of the age-restricted product by children and adolescents.
16 . The method of claim 15 , wherein the odorant composition is included in the age-restricted product at the EC 80 concentration.
17 . The method of claim 15 , wherein the odorant composition is included in the age-restricted product at the EC 85 concentration.
18 . The method of claim 15 , wherein the odorant composition is included in the age-restricted product at the EC 90 concentration.
19 . The method of claim 15 , wherein the one or more olfactory receptors is OR51E1, OR8K3, OR2W1, OR2J2, OR11H7P, OR5P3, or OR1G1.
20 . The method of claim 15 , wherein the age-restricted product comprises alcohol, cannabis, nicotine, or tobacco.
21 . The method of claim 15 , wherein the one or more olfactory receptors are part of a live cell assay.
22 . The method of claim 15 , wherein the one or more olfactory receptors are provided in a membrane fraction of a host cell.
23 . The method of claim 15 , wherein the one or more olfactory receptors are provided in liposomes.