Granted Patent
B2
US 12,351,862 · App. 17/576,550 · Granted Jul 8, 2025
Crisscross cooperative self-assembly
Inventors:
Dionis Minev (Cambridge, MA); Christopher Wintersinger (Cambridge, MA); William M. Shih (Cambridge, MA)
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Abstract
Provided herein, in some embodiments, are methods, compositions and kits for controlling nucleation and assembly of molecular nanostructures, microstructures and macrostructures.
Claims (16)
1. A crisscross nucleic acid nanostructure, comprising:
(a) a first plurality of deoxyribonucleic acid (DNA) nanorods aligned parallel to each other; and
(b) a second plurality of DNA nanorods aligned parallel to each other,
wherein the DNA nanorods of the first plurality are bound to and perpendicular to the DNA nanorods of the second plurality, a single DNA nanorod of (b) binds to multiple DNA nanorods of (a), each through a single cooperative binding site, and single DNA nanorod of (a) binds to multiple DNA nanorods of (b), each through a single cooperative binding site.
2. The crisscross nucleic acid nanostructure of claim 1 , wherein the DNA nanorods of the first plurality have a length of 10-500 nm and/or the DNA nanorods of the second plurality have a length of 10-500 nm.
3. The crisscross nucleic acid nanostructure of claim 1 , wherein the DNA nanorods of the first plurality comprise a 6-helix DNA bundle and/or the DNA nanorods of the second plurality comprise a 6-helix DNA bundle.
4. The crisscross nucleic acid nanostructure of claim 1 , wherein the first plurality comprises at least 4 DNA nanorods and/or the second plurality comprises at least 4 DNA nanorods.
5. The crisscross nucleic acid nanostructure of claim 1 , wherein the first plurality comprises at least 10 DNA nanorods and/or the second plurality comprises at least 10 DNA nanorods.
6. The crisscross nucleic acid nanostructure of claim 1 , wherein the first plurality comprises at least 25 DNA nanorods and/or the second plurality comprises at least 25 DNA nanorods.
7. The crisscross nucleic acid nanostructure of claim 1 , wherein the first plurality comprises at least 50 DNA nanorods and/or the second plurality comprises at least 50 DNA nanorods.
8. The crisscross nucleic acid nanostructure of claim 1 , wherein the cooperative binding site has a length of 5-50 nucleotides.
9. The crisscross nucleic acid nanostructure of claim 1 , wherein the nanostructure comprises 3-1000 cooperative binding sites.
10. The crisscross nucleic acid nanostructure of claim 9 , wherein the distance between each of the cooperative binding sites is 20-1000 angstroms.
11. A crisscross nucleic acid slat, comprising:
a first plurality of at least four DNA strands parallel to each other, each strand of the first plurality having a length of at least 21 nucleotides; and
a second plurality of at least four DNA strands parallel to each, each strand of the second plurality having a length of at least 21 nucleotides, wherein the at least four DNA strands of the first plurality are bound to and perpendicular to the at least four DNA strands of the second plurality.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 15, 2022
From: SHIH, WILLIAM M.
To: DANA-FARBER CANCER INSTITUTE, INC.
Reel/Frame 060513/0205 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 15, 2022
From: MINEV, DIONIS; WINTERSINGER, CHRISTOPHER
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 060513/0209 →
Continuity (3)
Continuation
16322787
Provisional Application
62370098
· Aug 2, 2016
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