Automated application of drift correction to sample studied under electron microscope
Methods and systems for calibrating a transmission electron microscope are disclosed. A fiducial mark on the sample holder is used to identify known reference points so that a current collection area and a through-hole on the sample holder can be located. A plurality of beam current and beam area measurements are taken, and calibration tables are extrapolated from the measurements for a full range of microscope parameters. The calibration tables are then used to determine electron dose of a sample during an experiment at a given configuration.
1. A method for measuring electron dose in a sample with a transmission electron microscope (TEM), the method comprising:
taking multiple measurements of the area of an electron beam of the TEM and the amount of current produced by the electron beam of the TEM,
wherein the TEM has different condenser lens settings for the multiple measurements of the area of the electron beam and the amount of current produced by the electron beam;
using the electron beam to excite the sample during an experiment performed on the sample using the TEM, wherein the TEM is set with particular condenser lens settings;
determining a beam area and a beam current of the electron beam used to excite the sample during the experiment for the particular condenser lens settings of the TEM based on the multiple measurements of the area of the electron beam and the amount of current produced by the electron beam; and
measuring an electron dose rate on the sample during the experiment based on the determined beam area and the determined beam current for the particular condenser lens settings of the TEM.
2. The method of claim 1 , wherein the area of the electron beam of the TEM is determined based on an image of the electron beam on a fluorescent screen of the TEM.
3. The method of claim 1 , wherein the area of the electron beam of the TEM is determined based on an image of the electron beam on a camera of the TEM.
4. The method of claim 1 , wherein the area of the electron beam of the TEM is determined based on one or more points identified at an edge of the electron beam.
5. The method of claim 1 , wherein the area of the electron beam of the TEM is determined using machine vision to identify the electron beam.
6. The method of claim 1 , wherein the amount of current produced by the electron beam of the TEM is determined using a current collector of the TEM, wherein the current collector of the TEM includes a fluorescent screen, a Faraday cup, or a TEM camera.
7. The method of claim 1 , wherein the multiple measurements are taken with the TEM set at different aperture settings for the electron beam.
8. The method of claim 1 , wherein the multiple measurements are taken with the TEM set at different acceleration voltage settings, convergence angles, emission currents, spot size, extraction voltages, or intensity settings for the electron beam.
9. The method of claim 1 , further comprising calculating the electron dose based on the measured electron dose rate at a specific area over a specific amount of time.
10. The method of claim 1 , wherein the electron dose on the sample is measured at a point in time during the experiment based on the determined beam area and the determined beam current for the particular condenser lens setting of the TEM during the point of time at which the electron dose is measured.
11. A microscope control system for measuring electron dose in a sample with a transmission electron microscope (TEM), the system comprising:
a processor configured for:
taking multiple measurements of the area of an electron beam of the TEM and the amount of current produced by the electron beam of the TEM,
wherein the TEM has different condenser lens settings for the multiple measurements of the area of the electron beam and the amount of current produced by the electron beam;
using the electron beam to excite the sample during an experiment performed on the sample using the TEM, wherein the TEM is set with particular condenser lens settings;
determining a beam area and a beam current of the electron beam used to excite the sample during the experiment for the particular condenser lens settings of the TEM based on the multiple measurements of the area of the electron beam and the amount of current produced by the electron beam; and
measuring an electron dose rate on the sample during the experiment based on the determined beam area and the determined beam current for the particular condenser lens settings of the TEM.
12. The microscope control system of claim 11 , wherein the area of the electron beam of the TEM is determined based on an image of the electron beam on a fluorescent screen of the TEM.
13. The microscope control system of claim 11 , wherein the area of the electron beam of the TEM is determined based on an image of the electron beam on a camera of the TEM.
14. The microscope control system of claim 11 , wherein the area of the electron beam of the TEM is determined based on one or more points identified at an edge of the electron beam.
15. The microscope control system of claim 11 , wherein the area of the electron beam of the TEM is determined using machine vision to identify the electron beam.
16. The microscope control system of claim 11 , wherein the amount of current produced by the electron beam of the TEM is determined using a current collector of the TEM, wherein the current collector of the TEM includes a fluorescent screen, a Faraday cup, or a TEM camera.
17. The microscope control system of claim 11 , wherein the multiple measurements are taken with the TEM set at different aperture settings for the electron beam.
18. The microscope control system of claim 11 , wherein the multiple measurements are taken with the TEM set at different acceleration voltage settings, convergence angles, emission currents, spot size, extraction voltages, or intensity settings for the electron beam.
19. The microscope control system of claim 11 , wherein the processor is further configured for calculating the electron dose based on the measured electron dose rate at a specific area over a specific amount of time.
20. The microscope control system of claim 11 , wherein the electron dose on the sample is measured at a point in time during the experiment based on the determined beam area and the determined beam current for the particular condenser lens setting of the TEM during the point of time at which the electron dose is measured.