METHODS OF IDENTIFYING AND SELECTING MAIZE PLANTS WITH RESISTANCE TO ANTHRACNOSE STALK ROT
Compositions and methods useful in identifying and/or selecting maize plants that have anthracnose stalk rot resistance are provided herein. The resistance may be newly conferred or enhanced relative to a control plant. The methods use maize markers on chromosome 10 to identify, select and/or construct resistant plants. Maize plants generated by the methods also provided.
1 . A method of obtaining a progeny maize plant comprising a marker allele associated with anthracnose stalk rot resistance, said method comprising:
a. providing a population of maize plants and isolating nucleic acids from each of the population of maize plants;
b. analyzing each of the isolated nucleic acids for the presence of a marker allele on chromosome 10 that is associated with anthracnose stalk rot resistance, wherein said marker allele comprises:
i. a “T” at sbd_INBREDA_4 at position 51 of SEQ ID NO: 6,
ii. a “C” at sbd_INBREDA_9 at position 51 of SEQ ID NO: 7,
iii. a “T” at sbd_INBREDA_13 at position 51 of SEQ ID NO: 8,
iv. a “T” at sbd_INBREDA_24 at position 51 of SEQ ID NO: 9,
v. a “T” at sbd_INBREDA_25 at position 51 of SEQ ID NO: 10,
vi. a “C” at sbd_INBREDA_32 at position 51 of SEQ ID NO: 11,
vii. an “A” at sbd_INBREDA_33 at position 51 of SEQ ID NO: 12, and
viii. a “G” at sbd_INBREDA_35 at position 51 of SEQ ID NO: 13;
c. selecting one or more maize plant in which said marker allele is detected;
d. crossing the selected one or more maize plants with one or more second maize plants to obtain a progeny plant comprising said marker allele.
2 . The method of claim 1 , wherein said marker allele is located in a chromosomal interval defined by and including markers C00429-801, wherein the marker comprises a “T” at position 84 of SEQ ID NO:1, and PHM824, wherein the marker comprises a “C” at position 278 of SEQ ID NO:4.
3 . The method of claim 1 , wherein said marker allele is located in a chromosomal interval defined by and including markers SYN17244, wherein the marker comprises a “T” at position 61 of SEQ ID NO:5, and sbd_INBREDA_48, wherein the marker comprises a “T” at position 51 of SEQ ID NO:14.
4 . The method of claim 1 , wherein said marker allele is located in a chromosomal interval defined by and including markers sbd_INBREDA_093, wherein the marker comprises a “A” at position 51 of SEQ ID NO:15, and sbd_INBREDA_109, wherein the marker comprises a “G” at position 51 of SEQ ID NO:16.
5 . The method of claim 1 , wherein said analyzing comprises isolating nucleic acids and detecting one or more marker alleles linked to and associated with said marker allele.
6 .- 15 . (canceled)
16 . Planting the progeny maize plant of claim 1 in a field having, or at risk for having, C. graminicola.
17 . The method of claim 1 , wherein the method comprises analyzing each of the isolated nucleic acids for the presence of a combination of two or more of the following marker alleles:
i. a “T” at sbd_INBREDA_4 at position 51 of SEQ ID NO: 6,
ii. a “C” at sbd_INBREDA_9 at position 51 of SEQ ID NO: 7,
iii. a “T” at sbd_INBREDA_13 at position 51 of SEQ ID NO: 8,
iv. a “T” at sbd_INBREDA_24 at position 51 of SEQ ID NO: 9,
v. a “T” at sbd_INBREDA_25 at position 51 of SEQ ID NO: 10,
vi. a “C” at sbd_INBREDA_32 at position 51 of SEQ ID NO: 11,
vii. an “A” at sbd_INBREDA_33 at position 51 of SEQ ID NO: 12, or
viii. a “G” at sbd_INBREDA_35 at position 51 of SEQ ID NO: 13.
18 . The method of claim 21 , wherein the method comprises analyzing each of the nucleic acids for the presence of each of marker alleles:
i. a “T” at sbd_INBREDA_4 at position 51 of SEQ ID NO: 6,
ii. a “C” at sbd_INBREDA_9 at position 51 of SEQ ID NO: 7,
iii. a “T” at sbd_INBREDA_13 at position 51 of SEQ ID NO: 8,
iv. a “T” at sbd_INBREDA_24 at position 51 of SEQ ID NO: 9,
v. a “T” at sbd_INBREDA_25 at position 51 of SEQ ID NO: 10,
vi. a “C” at sbd_INBREDA_32 at position 51 of SEQ ID NO: 11,
vii. an “A” at sbd_INBREDA_33 at position 51 of SEQ ID NO: 12, and
viii. a “G” at sbd_INBREDA_35 at position 51 of SEQ ID NO: 13.