Method for separation of antibodies or antibody fragments being devoid of an Fc region capable of binding to protein A
The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments.
1 . A method for separation of antibodies or antibody fragments, comprising the steps of:
a) providing a feed comprising antibodies or antibody fragments capable of binding to Protein A, where the antibodies or antibody fragments comprise a VH3 region and are devoid of an Fc region capable of binding to Protein A;
b) contacting said feed with a separation resin having ligands covalently coupled to a support, wherein said ligands consist essentially of a polypeptide as defined by SEQ ID NO 1:
AQX 1 AFYEILX 2 LPNLTEEQRX 3 AFIQSLKDDPSVSKAILAEAKKLNX 4
AQ
wherein:
X 1 =E, K, Y, T, F, L, W, I, M, V, A, H or R,
X 2 =H or K,
X 3 =N or A, and
X 4 =D, F, Y, W, K or R
and wherein said antibodies or antibody fragments bind to said separation resin;
c) optionally washing said separation resin with a washing liquid; and
d) eluting said antibodies or antibody fragments from said separation resin with an elution liquid and recovering said antibodies or antibody fragments.
2 . The method of claim 1 , wherein X 1 =E.
3 . The method of claim 1 , wherein X 2 =K.
4 . The method of claim 1 , wherein X 3 =N.
5 . The method of claim 1 , wherein X 4 =D.
6 . The method of claim 1 , wherein said ligands comprise multimers of said polypeptide, linked by linker regions comprising 0-15 amino acid residues.
7 . The method of claim 6 , wherein said multimers are tetramers, pentamers or hexamers.
8 . The method of claim 6 , wherein said multimers are coupled to said support via thioether links, derived from a C-terminal cysteine on said multimers.
9 . The method of claim 1 , wherein said support comprises crosslinked agarose beads.
10 . The method of claim 1 , wherein said support comprises crosslinked cellulose nanofibers.
11 . The method of claim 1 , wherein said feed is a clarified cell culture supernatant.
12 . The method of claim 1 , wherein said antibody fragments are selected from the group consisting of Fab, scFv, domain antibodies, nanobodies and BiTe.
13 . The method of claim 1 , wherein the binding strength of said antibody fragments to said separation resin is in the nanomolar range or stronger.
14 . The method of claim 1 , wherein said antibodies or antibody fragments are capable of binding to native Protein A via the VH3 region.
15 . The method of claim 1 , wherein the method comprises step c) and wherein contaminants and/or impurities are removed in step c).
16 . The method of claim 15 , wherein said washing liquid is a buffer of pH 5-7.
17 . The method of claim 1 , wherein said elution liquid is a buffer of pH 2-5.
18 . The method of claim 1 , further comprising, after step d), a step e) of cleaning said separation resin with a cleaning liquid of pH 13 or higher.
19 . The method of claim 18 , wherein said cleaning liquid comprises 0.5-2 M of an alkali metal hydroxide.
20 . The method of claim 18 , wherein steps a)-e) are repeated at least 50 times.