IP Library › Granted Patent US 12,527,801
Granted Patent B2
US 12,527,801 · App. 17/615,122 · Granted Jan 20, 2026

Cell activator of animal cell

Inventors: Hirokazu Kawagishi (Shizuoka, JP); Hisae Aoshima (Tokyo, JP)
Assignees: Vitamin C60 BioResearch Corporation; NATIONAL UNIVERSITY CORPORATION SHIZUOKA UNIVERSITY
A61K31/53A61K8/4966A61P17/16A61Q19/007A61Q19/02A61Q19/08C12N5/0629C12N2501/999
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Quick Facts
Patent No.
US 12,527,801
App. No.
17/615,122
Granted
Jan 20, 2026
Kind
B2
Abstract

A cell activator of an animal cell that includes 3H-imidazo[4,5-d][1,2,3]triazine-4,6(5H,7H)-dione.

Claims (8)

1 . A method for activating a human epidermal keratinocyte, the method comprising topically applying a composition that includes 3H-imidazo[4,5-d][1,2,3]triazine-4,6(5H,7H)-dione to skin of a human subject, wherein the activating is enhancing expression of at least one gene involved in one epidermal function selected from the group consisting of promoting cell-to-cell adhesion and barrier functions, stratum corneum exfoliation function, promoting differentiation and maturation, enhancing moisturizing action, and whitening, wherein the at least one gene is a gene selected from the group consisting of CLDN1, DSC1, DSG1, CDH1, KLK5, KLK7, SPIMK5, KRT1, KRT10, TGM1, IVL, SPRR1B, HAS3, FN1, and EDN1, and wherein the enhancing acts on at least one epidermal function selected from the group consisting of promoting turnover of an epidermis, promoting differentiation and maturation, promoting metabolism of old keratin, and enhancing a moisturizing action.

2 . The method according to claim 1 , wherein the human epidermal keratinocyte is a keratinocyte presenting in a stratum basale, a stratum spinosum, a stratum granulosum, or a stratum corneum of an epidermis of the human subject.

3 . A method for moisturizing skin, the method comprising topically applying a composition that includes 3H-imidazo[4,5-d][1,2,3]triazine-4,6(5H,7H)-dione to skin of a human subject.

4 . A method for promoting cell proliferation of a human epidermal keratinocyte, the method comprising topically applying a composition that includes 3H-imidazo[4,5-d][1,2,3]triazine-4,6(5H,7H)-dione to skin of a human subject.

5 . The method according to claim 4 , wherein the human epidermal keratinocyte is a keratinocyte presenting in a stratum basale, a stratum spinosum, a stratum granulosum, or a stratum corneum of an epidermis of the human subject.

6 . The method according to claim 1 , wherein the human epidermal keratinocyte is a keratinocyte presenting in a stratum corneum of an epidermis of the human subject.

7 . The method according to claim 3 , wherein the human epidermal keratinocyte is a keratinocyte presenting in a stratum corneum of an epidermis of the human subject.

8 . The method according to claim 4 , wherein the human epidermal keratinocyte is a keratinocyte presenting in a stratum corneum of an epidermis of the human subject.

Assignments (4)
MERGER Recorded Jan 13, 2026
From: VITAMIN C60 BIORESEARCH CORPORATION
To: MITSUBISHI CORPORATION LIFE SCIENCES LIMITED
Reel/Frame 074331/0669 →
MERGER Recorded Jan 13, 2026
From: VITAMIN C60 BIORESEARCH CORPORATION
To: MITSUBISHI CORPORATION LIFE SCIENCES LIMITED
Reel/Frame 074331/0764 →
MERGER Recorded Nov 25, 2025
From: VITAMIN C60 BIORESEARCH CORPORATION
To: MITSUBISHI CORPORATION LIFE SCIENCES LIMITED
Reel/Frame 073707/0167 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 30, 2021
From: KAWAGISHI, HIROKAZU; AOSHIMA, HISAE
To: VITAMIN C60 BIORESEARCH CORPORATION; NATIONAL UNIVERSITY CORPORATION SHIZUOKA UNIVERSITY
Reel/Frame 058235/0204 →
Priority Claims (1)
JP 2019-106031 · Jun 6, 2019 · national
Continuity (1)
Related Publication 20220233547A1 · Jul 28, 2022
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