Granted Patent
B2
US 12,702,718 · App. 17/623,144 · Granted Aug 11, 2026
Protein-enclosing polymeric micelle
Inventors:
Horacio Cabral (Tokyo, JP); Anqi Tao (Tokyo, JP); Kazunori Igarashi (Tokyo, JP); Takuya Miyazaki (Tokyo, JP); George Lo Huang (Tokyo, JP); Pengwen Chen (Tokyo, JP)
Assignee:
THE UNIVERSITY OF TOKYO
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Abstract
The present invention provides a polymeric complex comprising a protein and a block copolymer represented by the following formula (1).
Claims (23)
1 . A polymeric complex comprising a protein and a block copolymer represented by the following formula (1):
wherein R 1 represents an optionally substituted linear or branched alkyl group containing 1 to 12 carbon atoms and R 2 represents a hydrogen atom,
R 3 represents a compound represented by the following formula (Ia) or (Ib),
L 1 represents NH or a group represented by the following formula (11):
-(CH 2 ) p1 -NH- (11),
wherein p1 represents an integer of 1 to 6,
m1 and m2 each represent an integer of 0 to 500, provided that the sum of m1 and m2 represents an integer of 10 to 500, and with the proviso that m1 is not 0 and m2 is not 0,
m3, m4 and m5 each independently represent an integer of 1 to 5, and n represents an integer of 1 to 500, and
the symbol “/” means that (m1+m2) units of the respective monomer units shown on the left and right sides of this symbol may be in any sequence.
2 . The complex according to claim 1 , wherein the block copolymer represented by formula 1 is a block copolymer represented by the following formula (2),
3 . The complex according to claim 1 , wherein the protein is covalently bonded to the block copolymer represented by formula 1.
4 . The complex according to claim 3 , wherein the covalent bond is cleaved in a pH-dependent manner.
5 . A protein delivery device comprising the polymeric complex according to claim 1 for use in protein delivery to any site selected from a cell surface site, an intracellular site and an extracellular site.
6 . A protein delivery kit comprising a block copolymer represented by the following formula (1) for use in protein delivery to any site selected from a cell surface site, an intracellular site and an extracellular site:
wherein R 1 represents an optionally substituted linear or branched alkyl group containing 1 to 12 carbon atoms and R 2 represents a hydrogen atom,
R 3 represents a compound represented by the following formula (Ia) or (Ib),
L 1 represents NH or a group represented by the following formula (11):
—(CH 2 ) p1 -NH- (11)
wherein p1 represents an integer of 1 to 6,
m1 and m2 each independently represent an integer of 0 to 500 provided that the sum of m1 and m2 represents an integer of 1 to 500 and with the proviso that m1 is not 0 and m2 is not 0,
m3, m4 and m5 each independently represent an integer of 1 to 5, and n represents an integer of 1 to 500, and
the symbol “/” means that (m1+m2) units of the respective monomer units shown on the left and right sides of this symbol may be in any sequence.
7 . The kit according to claim 6 , wherein the block copolymer represented by formula 1 is a block copolymer represented by the following formula (2),
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 30, 2022
From: CABRAL, HORACIO; TAO, ANQI; IGARASHI, KAZUNORI; MIYAZAKI, TAKUYA; HUANG, GEORGE LO; CHEN, PENGWEN
To: THE UNIVERSITY OF TOKYO
Reel/Frame 059448/0001 →
Continuity (2)
Provisional Application
62868040
· Jun 28, 2019
Related Publication
20220249689A1
· Aug 11, 2022
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