Granted Patent
B2
US 12,213,995 · App. 17/628,011 · Granted Feb 4, 2025
Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use
Assignee:
DIRECT BIOLOGICS, LLC
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Abstract
Disclosed are compositions comprising a MSC secretome preparation and cannabinoids and methods of the use of said compositions for the treatment of inflammatory conditions.
Claims (11)
1. A composition comprising:
i) a mesenchymal stem cell (MSC) secretome preparation comprising MSC growth factors, MSC extracellular vesicles, MSC exosomes, and/or MSC extracts; and
ii) a cannabinoid;
wherein the MSC growth factors, MSC extracellular vesicles, MSC exosomes, and/or MSC extracts are obtained from human bone-marrow MSCs cultured under conditions comprising about 0.1% to about 5% oxygen and an acidic pH of 6.2 to 6.9.
2. The composition of claim 1 , wherein the cannabinoid is cannabidiol (CBD) or Cannabigerol (CBG).
3. A method of treating an inflammatory condition, an autoimmune disease, an autoinflammatory disease, metabolic disorder, or inflammation in a subject, the method comprising administering to the subject a therapeutically effective amount of the composition of claim 1 .
4. A method of treating an inflammatory condition, an autoimmune disease, an autoinflammatory disease, or metabolic disorder, in a subject, the method comprising culturing bone-marrow mesenchymal stem cells (MSCs) under conditions comprising about 0.1% to about 5% oxygen and an acidic pH of 6.2 to 6.9 to generate a mesenchymal stem cell (MSC) secretome preparation, and administering to the subject a therapeutically effective amount of the MSC secretome preparation and a cannabinoid.
5. The method of claim 4 , wherein the MSC secretome preparation and the cannabinoid are administered in the same composition.
6. The method of claim 4 , wherein the MSC secretome preparation is administered via inhalation.
7. The method of claim 4 , wherein the MSC secretome preparation and the cannabinoid are administered via inhalation.
8. A method of treating inflammation in a subject, the method comprising culturing bone-marrow mesenchymal stem cells (MSCs) under conditions comprising about 0.1% to about 5% oxygen and an acidic pH of 6.2 to 6.9 to generate a mesenchymal stem cell (MSC) secretome preparation and administering to the subject a therapeutically effective amount of the MSC secretome preparation and a cannabinoid.
Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 24, 2025
From: PETTINE, KENNETH ALLEN
To: DIRECT BIOLOGICS, LLC
Reel/Frame 072925/0519 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 27, 2025
From: PETTINE, KENNETH ALLEN
To: DIRECT BIOLOGICS, LLC
Reel/Frame 071550/0226 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 27, 2025
From: MOSELEY, TIMOTHY ALEXANDER
To: DIRECT BIOLOGICS, LLC
Reel/Frame 071741/0167 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 3, 2023
From: PETTINE, KENNETH ALLEN; MOSELEY, TIMOTHY ALEXANDER
To: DIRECT BIOLOGICS LLC
Reel/Frame 062583/0077 →
Continuity (3)
Provisional Application
62951905
· Dec 20, 2019
Provisional Application
62875889
· Jul 18, 2019
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