METHOD FOR PRODUCING EXOSOME

1 . A method for producing an exosome using a cell, the method comprising the steps of:

applying the cell to a cell culture module to culture the cell; and

allowing the cell to produce the exosome,

wherein the cell culture module comprises:

a porous polymer membrane; and

a casing having two or more medium flow inlets/outlets and containing the porous polymer membrane.

2 . The method according to claim 1 ,

wherein the porous polymer membrane is a three-layer structure porous polymer membrane having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B,

wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B,

wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B,

wherein the pores in the surface layers A and B communicate with the macrovoid, and

wherein the porous polymer membrane is contained within the casing with:

(i) the two or more independent porous polymer membranes being aggregated;

(ii) the porous polymer membranes being folded up;

(iii) the porous polymer membranes being wound into a roll-like shape; and/or

(iv) the porous polymer membranes being tied together into a rope-like shape.

3 . The method according to claim 1 or 2 , wherein the diameter of the medium flow inlet/outlet is larger than the diameter of the cell, and smaller than the diameter at which the porous polymer membranes flow out.

4 . The method according to any one of claims 1 to 3 , wherein the casing has a mesh-like structure.

5 . The method according to any one of claims 1 to 4 , wherein the casing consists of an inflexible material.

6 . The method according to any one of claims 1 to 5 , wherein the porous polymer membrane has a plurality of pores having an average pore diameter of 0.01 to 100 μm.

7 . The method according to any one of claims 2 to 6 , wherein an average pore diameter of the surface layer A is 0.01 to 50 μm.

8 . The method according to any one of claims 2 to 7 , wherein an average pore diameter of the surface layer B is 20 to 100 μm.

9 . The method according to any one of claims 1 to 8 , wherein a total membrane thickness of the porous polymer membrane is 5 to 500 μm.

10 . The method according to any one of claims 1 to 9 , wherein the porous polymer membrane is a porous polyimide membrane.

11 . The method according to claim 10 , wherein the porous polyimide membrane is a porous polyimide membrane comprising a polyimide derived from tetracarboxylic dianhydride and diamine.

12 . The method according to claim 10 or 11 , wherein the porous polyimide membrane is a colored porous polyimide membrane that is obtained by molding a polyamic acid solution composition comprising a polyamic acid solution derived from tetracarboxylic dianhydride and diamine, and a coloring precursor, and subsequently heat-treating the resultant composition at 250° C. or higher.

13 . The method according to any one of claims 1 to 12 , wherein the step of culturing a cell is performed under stationary culture conditions.

14 . The method according to any one of claims 1 to 12 , wherein the step of culturing a cell is performed under rotating or stirring culture conditions.

15 . The method according to any one of claims 1 to 14 , wherein the step of culturing a cell is performed continuously.

16 . The method according to any one of claims 1 to 15 ,

wherein the step of culturing a cell is performed in a cell culture device placed in an incubator, the cell culture device comprising:

a culture unit that contains the cell culture module configured to support the cell, and comprises a medium supply port and a medium discharge port; and

a culture medium supply unit comprising:

a culture medium storage container;

a medium supply line; and

a liquid-transfer pump configured to liquid-transfer a culture medium via the medium supply line, wherein a first end of the medium supply line is in contact with the culture medium in the culture medium storage container, and a second end of the medium supply line communicates with the culture unit via the medium supply port of the culture unit.

17 . The method according to claim 16 , wherein the cell culture device does not have the medium supply line, the liquid-transfer pump, an air supply port, an air discharge port, and an oxygen permeation membrane.

18 . The method according to any one of claims 1 to 17 , wherein the cell is selected from the group consisting of pluripotent stem cells, tissue stem cells, somatic cells, germ cells, sarcoma cells, established cell lines, and transformants.

19 . The method according to any one of claims 1 to 18 , wherein the cell is selected from the group consisting of human mesenchymal stem cells, osteoblasts, chondrocytes, and cardiomyocytes.

20 . The method according to any one of claims 1 to 19 , wherein the step of producing an exosome is at least partially the same as the step of culturing a cell.

21 . The method according to any one of claims 1 to 20 , wherein the step of producing an exosome is continued over 1 month, 2 months, 3 months, 6 months, or a longer period of time.

22 . An exosome production device for use in the method according to any one of claims 1 to 21 , the device comprising the cell culture module.

23 . A kit for use in the method according to any one of claims 1 to 21 , the device comprising the cell culture module.

24 . Use of the cell culture module for the method according to any one of claims 1 to 21 .

25 . An exosome obtained by the method according to any one of claims 1 to 21 , the method comprising the cell culture module.