Microorganisms for treatment of waste, water, or soil or for feeding to animals
The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus licheniformis MDG-1001 (NRRL No. B-67889), Bacillus subtilis/amyloliquefaciens MDG-8001 (NRRL No. B-67890), Bacillus pumilus MDG-1047 (NRRL No. B-67891), Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), Bacillus subtilis MDGV18 (NRRL No. B-67665), Bacillus pumilus MDGV17 (NRRL No. B-67664), Bacillus subtilis MDG-1728 (NRRL No. B-67618), Bacillus megaterium MDG-2705 (NRRL No. B-67619), and strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals.
1. A method of treating water, soil or waste by contacting the water, the soil or the waste with a Bacillus strain to remove a pollutant, the method comprising contacting the water, the soil or the waste with a composition comprising an effective amount of isolated Bacillus strains that express both cellulase and xylanase activity, said Bacillus strains comprising Bacillus megaterium MDG-2705, deposited as accession number NRRL No. B-67619, Bacillus licheniformis MDG-1000, deposited as accession number NRRL No. B-67888, and Bacillus licheniformis MDG-1001, deposited as accession number NRRL No. B-67889, wherein the Bacillus strains are in the form of a powder, a freeze-dried composition, a gel, or a pellet.
2. The method of claim 1 wherein the water or the waste is treated to remove a pollutant and the water or the waste is selected from the group consisting of industrial wastewater, industrial waste, residential wastewater, residential waste, agricultural wastewater, agricultural waste, and wastewater.
3. The method of claim 1 wherein removing the pollutant results in a reduction in odor or a reduction in chemical oxygen demand in the water, the waste, or the soil.
4. The method of claim 1 wherein the pollutant is an organic compound.
5. The method of claim 4 wherein the organic compound is removed by degradation.
6. The method of claim 1 wherein the pollutant is a synthetic compound.
7. The method of claim 1 wherein the pollutant is a paper-containing compound.
8. The method of claim 7 wherein the paper-containing compound is a wipe.
9. The method of claim 1 wherein the Bacillus strain produces an enzyme selected from the group consisting of an oxidoreductase, a hydrolase, a transferase, a lyase, an isomerase, a ligase, a peroxidase, a laccase, an esterase, an amylase, a protease, a xylanase, a lipase, a cellulase, an oxygenase, a reductase, an oxidase, a hydroxylase, a dehydrogenase, and combinations thereof.
10. The method of claim 1 further comprising contacting the water, the soil, or the waste with an exogenous enzyme selected from the group consisting of an oxidoreductase, a hydrolase, a transferase, a lyase, an isomerase, a ligase, a peroxidase, a laccase, an esterase, an amylase, a protease, a xylanase, a lipase, a cellulase, an oxygenase, a reductase, an oxidase, a hydroxylase, a dehydrogenase, and combinations thereof.
11. The method of claim 1 wherein the pollutant is a dye.
12. The method of claim 11 wherein the dye is an azo dye or an anthraquinone dye.
13. The method of claim 1 wherein said composition further comprises one or more Bacillus strains selected from the group consisting of Bacillus pumilus MDG-1047, deposited as accession number NRRL No. B-67891, Bacillus amyloliquefaciens MDG1607, deposited as accession number NRRL No. B-67666, Bacillus subtilis MDGV18, deposited as accession number NRRL No. B-67665, Bacillus pumilus MDGV17, deposited as accession number NRRL No. B-67664, Bacillus subtilis/amyloliquefaciens MDG-8001, deposited as accession number NRRL No. B-67890, and Bacillus subtilis strain MDG-1728, deposited as accession number NRRL No. B-67618.
14. The method of claim 11 wherein the pollutant is a sulfur dye.
15. The method of claim 1 wherein the pollutant is a dye and the Bacillus strain causes degradation or discoloration of the dye.
16. The method of claim 1 wherein the pollutant is a lignin.
17. The method of claim 16 wherein the lignin is produced by the paper industry or the pulp industry.
18. The method of claim 1 wherein the pollutant is an inorganic compound.
19. A commercial package comprising isolated Bacillus strains that express both cellulase and xylanase activity, said Bacillus strains comprising Bacillus megaterium MDG-2705, deposited as accession number NRRL No. B-67619 , Bacillus licheniformis MDG-1000, deposited as accession number NRRL No. B-67888, and Bacillus licheniformis MDG-1001, deposited as accession number NRRL No. B-67889, wherein the Bacillus strains are in the form of a powder, a freeze-dried composition, a gel, or a pellet.
20. The commercial package of claim 19 wherein said package further comprises one or more Bacillus strains selected from the group consisting of Bacillus pumilus MDG-1047, deposited as accession number NRRL No. B-67891 , Bacillus amyloliquefaciens MDG1607, deposited as accession number NRRL No. B-67666 , Bacillus subtilis MDGV18, deposited as accession number NRRL No. B-67665 , Bacillus pumilus MDGV17, deposited as accession number NRRL No. B-67664 , Bacillus subtilis/amyloliquefaciens MDG-8001, deposited as accession number NRRL No. B-67890, and Bacillus subtilis MDG-1728, deposited as accession number NRRL No. B-67618.
21. The commercial package of claim 20 wherein said one or more Bacillus strains comprise Bacillus subtilis MDG-1728, deposited as accession number NRRL No. B-67618.
22. The commercial package of claim 19 further comprising a binder.
23. The commercial package of claim 22 wherein the binder is selected from the group consisting of clay, yeast cell wall components, aluminum silicate, glucan, and combinations thereof.
24. The commercial package of claim 19 further comprising a cellulase.
25. The commercial package of claim 19 wherein said commercial package further comprises one or more Bacillus strains selected from the group consisting of Bacillus pumilus MDG-1047, deposited as accession number NRRL No. B-67891 and Bacillus subtilis MDG-1728, deposited as accession number NRRL No. B-67618.