SYSTEM
The present disclosure describes technologies that permit sensitive detection of nucleic acids of interest (i.e., nucleic acids whose nucleotide sequence is or includes a target sequence). Among other things the disclosure provides a system comprising: a plurality of nucleic acid molecules having different nucleotide sequences; a set of ligation oligonucleotides, comprising: a first ligation oligonucleotide whose nucleotide sequence includes a templating element and a first target hybridization element; and a second ligation oligonucleotide whose nucleotide sequence includes a second target hybridization element and optionally a second templating element; wherein the target hybridization elements bind to different portions of a common target site, to form a gapped nucleic acid strand susceptible to ligation with a ligase to generate a ligated strand that is amenable to lateral flow assessment.
1 . A system comprising:
a plurality of nucleic acid molecules having different nucleotide sequences;
a set of ligation oligonucleotides, comprising:
a first ligation oligonucleotide whose nucleotide sequence includes a templating element and a first target hybridization element; and
a second ligation oligonucleotide whose nucleotide sequence includes a second target hybridization element and optionally a second templating element; and
optionally one or more bridging oligonucleotides whose nucleotide sequence is or comprises one or more additional target hybridization elements,
wherein the target hybridization elements bind to different portions of a common target site, so that, when the plurality of nucleic acid molecules includes at least one nucleic acid molecule whose nucleotide sequence includes the target site, then the set of ligation oligonucleotides hybridizes to the target site and forms a gapped nucleic acid strand susceptible to ligation with a ligase to generate a ligated strand that is or generates a product amenable to lateral flow assessment.