SYSTEMS AND METHODS FOR DATA STORAGE USING NUCLEIC ACID MOLECULES
Disclosed herein are methods and systems for storing data and/or information on nucleic acid molecules, storing the nucleic acid molecules, and retrieving the data and/or information. These methods and systems have broad applications for data storage, including in improving the efficiency and accuracy of retrieving data.
1 .- 73 . (canceled)
74 . A method for storing data, comprising:
(a) encoding said data in a nucleic acid sequence;
(b) generating one or more linear nucleic acid molecules comprising at least a portion of said. nucleic acid sequences and circularizing said one or more linear nucleic acid molecules and amplifying by rolling circle amplification to generate one or more concatenated nucleic acid molecules; and
(c) storing said one or more linear nucleic acid molecules in an array disposed on a substrate, to provide said array when said array is imaged using an optical scanning system, wherein a wavelength of a signal generated from said one or more linear nuclei acid molecules or derivative thereof is greater than two times a size of a pixel of said optical scanning system.
75 . The method of claim 74 , wherein (b) comprises:
(a) generating one or more linear nucleic acid molecules that comprise said nucleic acid. sequence, a first adapter sequence, and a second adapter sequence, wherein said first and said second adapter sequence enable formation of one or more circular nucleic acid molecules; and
(b) amplifying said one or more circular nucleic acid molecules.
76 . The method of claim 75 , wherein said linear nucleic acid molecule comprises one or more functional sequences.
77 . The method of claim 75 , wherein said one or more circular nucleic acid molecules are generated by rolling circle amplification.
78 . The method of claim 77 , wherein (c) comprises disposing said one or more circular nucleic on said substrate.
79 . The method of claim 78 , wherein said one or more circular nucleic acid molecules are disposed at a density wherein an average distance between two or more nucleic acid molecules is less than a measure of λ/(2*NA), wherein λ is said wavelength of said signal generated from said one or more linear nucleic acids or derivatives thereof, and wherein NA is a numerical aperture of said optical scanning system.
80 . The method of claim 74 , further comprising retrieving said data from said one or more linear nucleic acid molecules without amplification prior to said retrieving.
81 . The method of claim 80 , wherein said retrieving comprises sequencing said one or more linear nucleic acid molecules or derivatives thereof.
82 . The method of claim 81 , wherein said sequencing comprises detecting one or more incorporated nucleic acids using said optical scanning system.
83 . A method for storing data, comprising disposing a nucleic acid molecule to a substrate, wherein said nucleic molecule or derivative thereof encodes said data, and wherein said data is retrieved from said nucleic acid molecule without amplification prior to sequencing.
84 . The method of claim 83 , wherein said nucleic acid molecule or derivative thereof comprises a nucleic acid concatemer.
85 . The method of claim 83 , wherein said nucleic acid molecule or derivative thereof is disposed at a density wherein when said substrate is imaged using an optical scanning system, a wavelength of a signal generated from said nucleic acid molecule or derivative thereof is greater than two times a size of a pixel of said optical scanning system.
86 . The method of claim 83 , wherein said substrate comprises silicon.
87 . The method of claim 83 , wherein said substrate comprises glass.
88 . The method of claim 83 , wherein said substrate comprises two pieces of glass.
89 . The method of claim 83 , wherein said nucleic acid molecule comprises a linear nucleic acid molecule.
90 . The method of claim 89 , wherein said linear nucleic acid molecule comprises one or more functional sequences.
91 . The method of claim 83 , wherein said data is retrieved by sequencing said one or more nucleic acid molecules or derivatives thereof.
92 . The method of claim 91 , wherein said sequencing comprises detecting one or more incorporate nucleic acids using a detection system.
93 . The method of claim 92 , wherein said detection system comprises an optical scanning system.