DEVICE AND METHOD FOR EVALUATING PROGRESSION OF DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO PIGMENT-CONTAINING CELLS
A device for evaluating progression of differentiation from pluripotent stem cells to pigment-containing cells includes a first irradiation unit configured to irradiate cells in a vessel with light in a melanin absorption wavelength band, a first detection unit configured to detect photo-acoustic waves generated from the cells irradiated with the light, and a processor configured to output an evaluation result relevant to the progression of the differentiation of the cells to the pigment-containing cells, based on intensity of the detected photo-acoustic waves detected by the first detection unit.
1 . A device for evaluating progression of differentiation from pluripotent stem cells to pigment-containing cells, the device comprising:
a first irradiation unit configured to irradiate cells in a vessel with light in a melanin absorption wavelength band;
a first detection unit configured to detect photo-acoustic waves generated from the cells irradiated with the light; and
a processor,
wherein the processor is configured to output an evaluation result relevant to the progression of the differentiation of the cells to the pigment-containing cells, based on intensity of the photo-acoustic waves detected by the first detection unit.
2 . The device according to claim 1 ,
wherein the first irradiation unit includes a scanning unit configured to scan the cells with the light,
the processor is configured to output a photo-acoustic image based on the intensity of the photo-acoustic waves detected by the first detection unit and an irradiation position of the light when detecting the photo-acoustic waves with the intensity.
3 . The device according to claim 2 , further comprising:
a second irradiation unit configured to irradiate the cells with illumination light; and
a second detection unit configured to detect observation light from the cells irradiated with the illumination light,
wherein the processor is configured to output a superimposed image in which a cell image based on the observation light detected by the second detection unit is superimposed on the photo-acoustic image.
4 . The device according to claim 1 , further comprising:
a second irradiation unit configured to irradiate the cells with illumination light; and
a second detection unit configured to detect observation light from the cells irradiated with the illumination light,
wherein the first irradiation unit includes a scanning unit configured to scan the cells with the light, and
the evaluation result includes differentiation rate information relevant to a rate of differentiated cells in the vessel to the total cells in the vessel, which is calculated on the basis of the intensity of the photo-acoustic waves detected by the first detection unit, an irradiation position of the light when detecting the photo-acoustic waves with the intensity, and a cell image based on the observation light detected by the second detection unit.
5 . The device according to claim 1 , further comprising:
a second irradiation unit configured to irradiate the cells with illumination light; and
a second detection unit configured to detect observation light from the cells irradiated with the illumination light,
wherein the first irradiation unit includes a scanning unit scanning the cells with the light, and
the evaluation result includes progression degree information relevant to a differentiation progression degree of each of the cells, which is calculated on the basis of the intensity of the photo-acoustic waves detected by the first detection unit, an irradiation position of the light when detecting the photo-acoustic waves with the intensity, and a cell image based on the observation light detected by the second detection unit.
6 . The device according to claim 5 ,
wherein the processor is configured to output a second superimposed image in which a position image representing positions of undifferentiated cells specified on the basis of the progression degree information is superimposed on the cell image.
7 . The device according to claim 3 ,
wherein the second detection unit includes a phase film for a phase contrast observation.
8 . The device according to claim 4 ,
wherein the second detection unit includes a phase film for a phase contrast observation.
9 . The device according to claim 5 ,
wherein the second detection unit includes a phase film for a phase contrast observation.
10 . The device according to claim 6 ,
wherein the second detection unit includes a phase film for a phase contrast observation.
11 . The device according to claim 3 ,
wherein the second irradiation unit is configured to irradiate the cells with illumination light from a direction intersecting with an optical axis of an objective lens to be included in the second detection unit.
12 . The device according to claim 4 ,
wherein the second irradiation unit is configured to irradiate the cells with illumination light from a direction intersecting with an optical axis of an objective lens to be included in the second detection unit.
13 . The device according to claim 5 ,
wherein the second irradiation unit is configured to irradiate the cells with illumination light from a direction intersecting with an optical axis of an objective lens to be included in the second detection unit.
14 . The device according to claim 6 ,
wherein the second irradiation unit is configured to irradiate the cells with illumination light from a direction intersecting with an optical axis of an objective lens to be included in the second detection unit.
15 . The device according to claim 1 ,
wherein a center wavelength of the light is included in a range of 600 nm to 800 nm.
16 . The device according to claim 1 ,
wherein the evaluation result includes information relevant to an amount of melanin to be contained in the cells, which is estimated on the basis of the intensity of the photo-acoustic waves.
17 . The device according to claim 1 ,
wherein the vessel is a culture vessel, and
the culture vessel includes at least one of the a bioreactor, a culture bag, a flask, a dish, and a well plate.
18 . The device according to claim 1 ,
wherein the pigment-containing cells include melanocyte.
19 . The device according to claim 1 ,
wherein the pigment-containing cells include retinal pigmentary epithelial cells.
20 . A method for evaluating progression of differentiation from pluripotent stem cells to pigment-containing cells, the method comprising:
irradiating cells in a vessel with light in a melanin absorption wavelength band;
detecting photo-acoustic waves generated from the cells irradiated with the light; and
outputting an evaluation result relevant to the progression of the differentiation of the cells to the pigment-containing cells, based on intensity of the detected photo-acoustic waves.