Method, computer program product, and microscopy system for displaying polarizing samples
The invention relates to a method for displaying polarizing samples, a microscopy system, and a computer program product. According to the invention, different types of polarization contrasts are to be prepared and provided for a common, simultaneous display on a user interface, wherein the same polarization angle is automatically displayed for all contrast types. If the selected polarization angle is changed, it should be changed synchronously in the display of all contrast types.
1. A method for displaying polarizing samples, with at least the following steps:
arranging and aligning a sample in an observation system,
acquiring at least two shots with at least one polarization contrast and different polarization angles,
grouping the shots according to their polarization contrast type, and
preparing and providing the images of all available groupings for simultaneous display on a user interface with one image each for the same polarization angle.
2. The method according to claim 1 , wherein further shots are acquired and grouped without polarization contrast.
3. The method according to claim 1 , wherein, by actuating a control element provided for this purpose on the user interface, it is possible to switch from a display with a first polarization angle to a display with a second polarization angle, wherein the switch is displayed synchronously for each available grouping.
4. The method according to claim 1 , wherein an interpolated image is displayed, in particular an image generated by means of trigonometric interpolation, to provide an image for which there is no shot with the selected polarization angle.
5. The method according to claim 1 , wherein, by actuating the control element for changing the selected polarization angle, a synchronously rotated display of the images of all available groupings is provided at the same time.
6. The method according to claim 1 , wherein an orientation element is provided for display on the user interface, which element displays the selected polarization angle.
7. The method according to claim 1 , wherein only previously selected groupings are provided for display on the user interface.
8. A non-transitory computer-readable storage medium storing a computer program product containing instructions for carrying out the method according to claim 1 .
9. The method according to claim 1 , wherein the acquiring the at least two shots with the at least one polarization contrast and different polarization angles includes acquiring at least two shots for each of at least two polarization contrasts and different polarization angles.
10. A microscopy system for displaying polarizing samples, in particular with a method according to claim 1 , comprising:
an observation system designed to acquire at least two shots of a sample with at least one polarization contrast and different polarization angles,
a computing unit designed and configured for grouping the shots according to their polarization contrast type, as well as for the preparation and provision thereof for a display device, and
a display device, on which a user interface is provided and on which all available groupings are displayed simultaneously with one image each with the same polarization angle.
11. The microscopy system according to claim 10 , wherein it has a rotatable table, a rotatable polarizer, a rotatable analyzer, a filter wheel with differently aligned analyzers, and/or an on-chip polarizer in order to acquire the shots with different polarization angles.
12. The method according to claim 9 , wherein the at least two polarization contrasts are selected from cross polarization, plane-parallel polarization, circular polarization, and pleochroic polarization.