Granted Patent B2

US 12,564,578 · App. 17/682,856 · Granted Mar 3, 2026

Inventors: Ren Xu (Lexington, KY); Shike Wang (Lexington, KY)

Assignee: University of Kentucky Research Foundation

A61K31/4365A61K31/216A61K31/337A61K31/404G01N33/582

Abstract

Claims (10)

1 . A method of screening for modulators of C-P4H1, comprising:

(a) expressing a P4H1 complex in a eukaryotic cell;

(b) purifying the P4H1 complex;

(c) adding the purified P4H1 complex to a well of a high throughput assay plate;

(d) adding peptide substrate GlyProProGlyOEt, FeSO 4 , catalase, ascorbate, and α-ketoglutarate to the well of the high throughput assay plate;

(e) adding a test molecule or control to the well of the high throughput assay plate;

(f) adding 3-oxoacid CoA-transferase (SCOT) and succinyl CoA ligase (SCS), in the presence of acetoacetyl-CoA to convert succinate into ATP to the well of the high throughput assay plate;

(g) adding luciferase to convert ATP into light energy to the well of the high throughput assay plate;

(h) measuring luminescence of the well of the high throughput assay plate; and

(i) identifying a molecule as a modulator of P4H1 when the molecule increases luminescence of the well relative to negative control or decreases luminescence of the well relative to positive control.

Assignments (1)

Continuity (3)

References Cited (28)