Optical reader for analyte testing
Analyte collection and testing systems and methods, and more particularly to disposable oral fluid collection and testing systems and methods. Described herein are methods and apparatuses to achieve significant improvements in the detection of fluorescence signals in the reader.
1. A method of reading optical signals from a photonic chip of a removable cartridge in an optical reader, the method comprising:
aligning a scan head of the optical reader with the chip so that a laser source, a plurality of fibers, and an optical sensor of the scan head are aligned along a polarization axis with the chip;
maintaining a polarization of the polarization axis in a transverse-magnetic (TM) polarization; and
emitting one or more beams of light from the laser source, through the plurality of fibers and into an edge of the photonic chip in the TM polarization; and
detecting, in the optical sensor, TM polarized light from one or more waveguides within the chip when the one or more beams of light interact with an analyte molecule on the chip.
2. The method of claim 1 , further comprising inserting the cartridge containing the chip into the optical reader prior to aligning the scan head.
3. The method of claim 1 , wherein emitting comprises emitting a plurality of concurrent beams of TM polarized light from the scan head, into the edge of the photonic chip.
4. The method of claim 1 , wherein maintaining the polarization of the polarization axis comprises maintaining the polarization of the plurality of fibers.
5. The method of claim 1 , further comprising polarizing light emitted from the scan head to the edge of the chip in a polarizer.
6. The method of claim 1 , further comprising adjusting the alignment of the scan head while emitting and/or detecting to maintain the TM polarization.
7. An optical reader device for reading a photonic chip of a removable cartridge, the device comprising:
a scan head;
a plurality of laser sources each configured to emit light having a transverse-magnetic (TM) polarization;
a first plurality of optical fibers, wherein each laser source is coupled to one optical fiber of the first plurality of optical fibers, further wherein the first plurality of optical fibers are polarization maintaining single-mode fibers;
a plurality of optical sensors;
a second plurality of optical fibers, wherein each optical sensor is coupled to one optical fiber of the second plurality of optical fibers, further wherein the second plurality of optical fibers are multimode fibers;
wherein each of the first plurality of optical fibers and the second plurality of optical fibers terminates on the scan head so that an end of each optical fiber of the first and second pluralities of optical fibers are arranged in a line facing a gap; and
a cartridge holder configured to receive the removable cartridge so that the photonic chip is aligned with a polarization axis formed by the scan head so that an end of the photonic chip comprising a plurality of waveguides faces the gap, across from the scan head,
wherein the device is configured to maintain the polarization of the polarization axis in a transverse-magnetic (TM) polarization.
8. The device of claim 7 , wherein the laser sources comprise diode lasers.
9. The device of claim 7 , wherein the second plurality of fibers comprises at least twice as many optical fibers as the first plurality of fibers.
10. The device of claim 7 , further comprising a controller configured to control alignment of the scan head relative to the cartridge.
11. The device of claim 7 , further wherein the cartridge holder is configured to clamp the cartridge to prevent it from moving.
12. The device of claim 7 , wherein the cartridge holder is configured to bias the cartridge in normal to a major plane of the cartridge against a reference surface to prevent movement of the cartridge as one or more actuators apply force to the cartridge to drive fluid through the cartridge.
13. The device of claim 10 , wherein the controller is configured to adjust a position of the scan head during operation of the device by actuating a scan head actuator to align the ends of the optical fibers with waveguides of the photonic chip when the cartridge is in the cartridge holder.