Cryostorage device
Disclosed herein are cryostorage devices, systems, and methods for cryopreservation or vitrification of biological materials, such as oocytes and embryos. These cryostorage devices can include a capillary straw with the dual functionality for loading/unloading a sample. The devices can also include a self-sealing mechanism and an adapter for coupling with pipettes to enable loading of a predetermined volume of sample. The devices can also include a removable cap to protect the capillary during long-term cryostorage. Methods described herein relate to the manual or automated use of such devices.
1. A cryostorage device, comprising:
a) a capillary element that comprises an elongated hollow portion between a bottom opening and a top opening; and
b) a hollow body that comprises a lower end, an upper end, and a self-sealing valve, wherein the self-sealing valve is at the upper end, wherein the hollow body is connected to the top opening of the elongated hollow portion of the capillary element through the lower end of the hollow body.
2. The cryostorage device of claim 1 , wherein the hollow body is comprised of a material that is freeze tolerant.
3. The cryostorage device of claim 1 , wherein the hollow body is comprised of an elastomeric material.
4. The cryostorage device of claim 1 , wherein the hollow body is configured to aspirate a sample into the cryostorage device through the capillary element.
5. The cryostorage device of claim 1 , wherein the hollow body is configured to expel a sample from the cryostorage device through the capillary element.
6. The cryostorage device of claim 1 , wherein the self-sealing valve is configured to release excess pressure from the capillary element.
7. The cryostorage device of claim 1 , further comprising a pipette, wherein the pipette is operably connected to the upper end of the hollow body such that the pipette actuates the cryostorage device.
8. The cryostorage device of claim 7 , wherein the pipette is a micropipette.
9. The cryostorage device of claim 7 , wherein the pipette is a robotized pipette.
10. The cryostorage device of claim 1 , further comprising a cylindrical tube, wherein the cylindrical tube connects to the hollow body and connects to the capillary element, wherein the cylindrical tube partially shrouds the capillary element at the top opening of the capillary element, wherein the cylindrical tube is configured to reinforce the capillary element.
11. The cryostorage device of claim 1 ; further comprising a detachable protective cover that comprises an opened end and a closed end, wherein the cover is connected to the hollow body at the opened end and shrouds the capillary element.
12. The cryostorage device of claim 1 , further comprising a pipette adapter, wherein the pipette adapter is operably connected to the hollow body of the cryostorage device and a pipette, wherein the pipette adapter comprises:
a) a lower end that comprises a conical peak, wherein the lower end is configured to receive the hollow body;
b) an upper end that is connected to a cavity within the pipette adapter, wherein the upper end is configured to receive the pipette; and
c) a channel within the conical peak, wherein the hollow body and the cavity are fluidically connected through the channel, wherein the conical peak opens the self-sealing valve of the hollow body when the hollow body is coupled to the adapter.
13. The cryostorage device of claim 12 , wherein self-sealing valve connects to the conical peak of the lower end of the pipette adapter, wherein the self-sealing valve reduces a likelihood of leakage of a liquid sample contained within the cryostorage device.
14. A pipette adapter for coupling a cryostorage device and a pipette, comprising:
a) a lower end configured to receive the cryostorage device, wherein the lower end comprises a conical peak and a concentric recess that surrounds the conical peak;
b) an upper end configured to receive the pipette, wherein the upper end comprises a cavity; and
c) a channel within the conical peak, wherein the channel fluidically connects the conical peak of the lower end and the cavity of the upper end.
15. The pipette adapter of claim 14 , wherein the cryostorage device comprises a self-sealing valve, wherein self-sealing valve connects to the conical peak of the lower end of the pipette adapter, wherein the self-sealing valve reduces a likelihood of leakage of a liquid sample contained within the cryostorage device.