IP Library › Granted Patent US 12,551,506
Granted Patent B2
US 12,551,506 · App. 17/728,956 · Granted Feb 17, 2026

Differentiation of olfactory mucosa derived mesenchymal stem cells to schwann cells for peripheral nerve repair

Inventors: Ryan A. Koppes (Charlestown, MA); Katelyn E. Neuman (Boston, MA); Abigail N. Koppes (Charlestown, MA); Aidan Kenny (Boston, MA)
Assignee: NORTHEASTERN UNIVERSITY
A61K35/30A61P25/00C12N5/0622C12N2500/32C12N2500/44C12N2501/13C12N2506/1392
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Quick Facts
Patent No.
US 12,551,506
App. No.
17/728,956
Granted
Feb 17, 2026
Kind
B2
Abstract

Provided herein are, in various embodiments, methods and compositions for differentiating olfactory mucosa-derived mesenchymal stem cells (OM-MSC). In certain embodiments, the disclosure provides for media to differentiate OM-MSCs. In still further embodiments, the disclosure provides for methods and compositions using differentiated OM-MSCs for the treatment of nerve repair. In particular embodiments, the disclosure provides for novel treatments of peripheral nerve repair.

Claims (22)

1 . A method of promoting neurite outgrowth in a neuron, the method comprising:

a) propagating olfactory mucosa-derived mesenchymal stem cells (OM-MSCs) in Schwann cell conditioned media (SCCM) for about 14-21 days, thereby producing differentiated olfactory mucosa-derived mesenchymal stem cells (dOM-MSCs);

b) isolating the-dOM-MSCs, thereby producing isolated dOM-MSCs; and

c) contacting the neuron with a composition comprising the isolated dOM-MSCs, thereby promoting neurite outgrowth in the neuron.

2 . The method of claim 1 , wherein the SCCM comprises:

a) Dulbecco's Modified Eagle Medium (DMEM);

b) about 10% v/v fetal bovine serum (FBS);

c) about 50 U/mL antibiotic, wherein the antibiotic is penicillin or streptomycin, or a combination thereof;

d) about 2 mM of L-glutamine;

e) about 0.01-10 μg/mL bovine pituitary extract (BPE); and

f) about 0.06-6.6 μM Forskolin.

3 . The method of claim 2 , wherein the SCCM further comprises at least one growth factor selected from Nerve Growth Factor (NGF), Brain Derived Neurotropic Factor (BDNF), Glial-cell Derived Neurotropic Factor (GDNF), or a combination thereof.

4 . The method of claim 3 , wherein a concentration of at least one growth factor is:

a) about 0.0025-2.5 ng/mL Nerve Growth Factor (NGF);

b) about 0.003-3 ng/mL Brain Derived Neurotropic Factor (BDNF); and/or

c) about 0.0021-2.1 μg/mL Glial-cell Derived Neurotropic Factor (GDNF).

5 . The method of claim 1 , wherein the composition comprising the isolated dOM-MSCs further comprises a nerve guide conduit.

6 . The method of claim 1 , wherein the neuron is a damaged neuron, and promoting neurite outgrowth in the neuron repairs the damaged neuron.

7 . The method of claim 1 , wherein the neuron is in a human subject.

8 . The method of claim 1 , wherein the OM-MSCs are from a human subject.

9 . The method of claim 1 , wherein the dOM-MSCs have increased neuroepithelial stem cell protein (Nestin) expression compared to OM-MSCs or OM-MSCs differentiated by chemical induction.

10 . The method of claim 1 , wherein the dOM-MSCs have decreased actin filament expression compared to OM-MSCs or OM-MSCs differentiated by chemical induction.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 20, 2022
From: KOPPES, RYAN A.; NEUMAN, KATELYN E.; KOPPES, ABIGAIL N.; KENNY, AIDAN
To: NORTHEASTERN UNIVERSITY
Reel/Frame 059975/0674 →
Continuity (2)
Provisional Application 63178527 · Apr 23, 2021
Related Publication 20220339200A1 · Oct 27, 2022
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