Substituted [1,2,4]triazolo[4,3-c]pyrimidin-5-amines and proteolysis-targeting chimeric derivatives thereof (PROTACs) that induce degradation of embryonic ectoderm development (EED) protein
Disclosed are compounds that bind to embryonic ectoderm development (EED) protein and proteolysis-targeting chimeric (PROTAC) derivatives thereof that induce degradation of EED. The disclosed compounds may be characterized as substituted [1,2,4]triazolo[4,3-c]pyrimidin-5-amine compounds. The disclosed PROTAC derivatives thereof typically include a first targeting moiety that binds to EED (M EED ) which may be derived from the disclosed [1,2,4]triazolo[4,3- c ]pyrimidin-5-amine compounds that bind to EED. The first targeting moiety typically is linked via a bond or a linker (L) to a second targeting moiety that binds to an E3 ubiquitin ligase (M E3 ). As such, the disclosed PROTACS may be described as having a formula M EED -L-M E3 or M E3 -L-M EED , wherein M EED has a formula of where R 2 , n, and x are as described herein.
1. A compound of the following formula:
M EED -L-M E3
or a pharmaceutically acceptable salt or stereoisomer thereof,
wherein:
M EED is Formula II:
wherein:
n is 1;
x is 1;
R 2 is a bond or —C(O)—; and
* is the point of attachment to the —CH 2 — of the —(CH 2 CH 2 O) n — of L;
(i) L is a linker of the following formula:
*—(CH 2 CH 2 O) n CH 2 CH 2 -+
wherein:
n is 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20;
* is the point of attachment to the piperazinylene nitrogen atom or —C(O)— of M EED ; and
+ is the point of attachment to the —C(O)—, —NH—, or —O— of M E3 ; or
(ii) L is a linker of the following formula:
*—(CH 2 CH 2 O) n CH 2 CH 2 C(O)NHCH 2 CH 2 -+
wherein:
n is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20;
* is the point of attachment to the piperazinylene nitrogen atom or —C(O)— of M EED ; and
+ is the point of attachment to the —C(O)—, —NH—, or —O— of M E3 ; and
M E3 is:
wherein:
+ is the point of attachment to the —CH 2 — of L.
2. The compound of claim 1 , or a pharmaceutically acceptable salt or stereoisomer thereof, wherein M EED is of the following formula:
wherein:
* is the point of attachment to the —CH 2 — of the —(CH 2 CH 2 O) n — of L.
3. The compound of claim 1 , or a stereoisomer thereof, wherein the compound, or stereoisomer thereof, is selected from the group consisting of:
or a pharmaceutically acceptable salt thereof.
4. A pharmaceutical composition comprising a pharmaceutically acceptable carrier, excipient, or diluent and a compound of claim 1 , or a pharmaceutically acceptable salt or stereoisomer thereof.