Use of stereoselective transaminase in asymmetric synthesis of chiral amine
Use of a stereoselective transaminase in the asymmetric synthesis of a chiral amine. In particular, provided is use of a polypeptide in the production of a chiral amine or a downstream product using a chiral amine as a precursor. Further provided is a method for producing a chiral amine, comprising culturing a strain expressing the polypeptide so as to obtain a chiral amine. Further provided are novel prochiral compounds, a chiral amine production strain and a method for constructing the chiral amine production strain. The stereoselective transaminase has a broad substrate spectrum and thus has a broad application potential in the preparation of a chiral amine.
1. A compound of Formula II, a pharmaceutically acceptable salt thereof, or a stereoisomer thereof:
wherein,
R 1 is selected from the group consisting of none, oxo (═O), halogen, —OH, —NH 2 , substituted or unsubstituted C1-C 8 alkyl, substituted or unsubstituted C 3 -C 8 cycloalkyl, substituted or unsubstituted C 1 -C 8 alkoxy, substituted and unsubstituted C 3 -C 8 cycloalkoxy;
R 2 is selected from the group consisting of none, halogen, —OH, —NH 2 , substituted or unsubstituted C 1 -C 8 alkyl, substituted or unsubstituted C 3 -C 8 cycloalkyl, substituted or unsubstituted C 1 -C 8 alkoxy, substituted and unsubstituted C 3 -C 8 cycloalkoxy;
X is selected from the group consisting of: C and N;
Y is selected from the group consisting of: C, N, O, and S;
wherein R 2 is none when Y is O or S.
2. The compound of claim 1 , the pharmaceutically acceptable salt thereof, or the stereoisomer thereof, wherein the compound of Formula II comprises the following compounds: