Modified 3D-printed objects and their uses
Provided herein are methods which alter the mechanical and biological properties of polymeric materials. Also provided are compositions comprising the polymeric materials having said properties.
1. A method of modifying a polymeric scaffold comprising polymerized poly(ethyelene glycol) di(meth)acrylate moieties, polymerized poly(ethyelene glycol) di(meth)acrylamide moieties, polymerized poly(ethyelene glycol) (meth)acrylate/(methacrylamide) moieties, or mixtures thereof, the method comprising:
providing the polymeric scaffold;
contacting the polymeric scaffold with a hydrolysis agent comprising NaOH and a proteolysis agent comprising cholesterol esterase; and
after contacting the polymeric scaffold with the hydrolysis agent and the proteolysis agent, contacting the polymeric scaffold with lung cells,
wherein contacting the polymeric scaffold with the hydrolysis agent and the proteolysis agent increases lung cell attachment to the polymeric scaffold and reduces a Young's modulus of the polymeric scaffold.
2. The method of claim 1 , wherein the polymeric scaffold comprises polymerized poly(ethyelene glycol) diacrylate moieties.
3. The method of claim 2 , wherein the polymerized poly(ethyelene glycol) diacrylate moieties comprise PEGDA3400, PEGDA575, or a mixture thereof.
4. The method of claim 1 , wherein the polymeric scaffold further comprises polymerized collagen.
5. The method of claim 1 , wherein the polymeric scaffold further comprises polymerized hydroxypropyl acrylate (HPA).
6. The method of claim 1 , wherein the polymeric scaffold further comprises a polymerized UV initiator.
7. The method of claim 1 , wherein the concentration of the hydrolysis agent is about 1 mM to about 25 mM, about 25 mM to about 50 mM, about 50 mM to about 100 mM, about 100 mM to about 150 mM, about 150 mM to about 300 mM, about 300 mM to about 500 mM, about 500 mM to about 1 M, about 1 M to about 5 M, or about greater than 5 M.
8. The method of claim 1 , wherein the scaffold is contacted with the hydrolysis agent for about 1 min to about 30 min, about 30 min to about 1 hr, about 1 hr to about 2.5 hr, about 2.5 hr to about 5 hr, about 5 hr to about 7.5 hr, about 7.5 hr to about 10 hr, about 10 hr to about 24 hr, about 24 hr to about 2 days, about 2 days to about 4 days, about 4 days to about 8 days, about 8 days to about 12 days, about 12 days to about 30 days, or greater than about 30 days.
9. The method of claim 1 , wherein the concentration of the proteolysis agent is about 0.1 U/mL to about 1 U/mL, about 1 U/mL to about 2.5 U/mL, about 2.5 U/mL to about 5 U/mL, about 5 U/mL to about 7.5 U/mL, about 7.5 U/mL to about 10 U/mL, about 10 U/mL to about 15 U/mL, or about greater than 15 U/mL.
10. The method of claim 1 , wherein the scaffold is contacted with the proteolysis agent for about 1 hr, about 1 hr to about 2.5 hr, about 2.5 hr to about 5 hr, about 5 hr to about 7.5 hr, about 7.5 hr to about 10 hr, about 10 hr to about 24 hr, about 24 hr to about 2 days, about 2 days to about 4 days, about 4 days to about 8 days, about 8 days to about 12 days, about 12 days to about 30 days, or greater than about 30 days.
11. The method of claim 1 , wherein the scaffold is a 3D-printed scaffold.
12. The method of claim 1 , wherein the scaffold comprises channels and walls.
13. The method of claim 12 , wherein the channels have a width of about 200 μm to about 500 μm.
14. The method of claim 12 , wherein the walls have a width of about 150 μm to about 400 μm.
15. A polymeric scaffold produced by the method of claim 1 .
16. A method of increasing the affinity of a polymeric scaffold for cells, the polymeric scaffold comprising polymerized poly(ethyelene glycol) di(meth)acrylate moieties, polymerized poly(ethyelene glycol) di(meth)acrylamide moieties, polymerized poly(ethyelene glycol) (meth)acrylate/(methacrylamide) moieties, or mixtures thereof,
wherein the method comprises providing the polymeric scaffold and contacting the polymeric scaffold with a hydrolysis agent comprising NaOH and a proteolysis agent comprising cholesterol esterase; and
after contacting the polymeric scaffold with the hydrolysis agent and the proteolysis agent, contacting the polymeric scaffold with lung cells,
wherein contacting the polymeric scaffold with the hydrolysis agent and the proteolysis agent increases lung cell attachment to the polymeric scaffold and reduces a Young's modulus of the polymeric scaffold.