Fluidic devices including fluidic channels, and methods of making the same
In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.
1. A method of preparing a fluidic channel, the method comprising:
covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover,
wherein the covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel; further comprising disposing an intervening layer between the first region of the substrate and the first region of the cover, wherein the covalently coupling the first region of the substrate to the first region of the cover comprises: covalently coupling the first region of the substrate to the intervening layer; and covalently coupling the first region of the cover to the intervening layer, wherein the covalently coupling the first region of the substrate to the intervening layer comprises a first azide-alkyne [3+2] cyclo-addition.
2. The method of claim 1 , further comprising coupling oligonucleotides to the second region of the substrate.
3. The method of claim 2 , wherein the oligonucleotides are coupled to the second region of the substrate before the first region of the substrate is coupled to the first region of the cover.
4. The method of claim 2 , further comprising protecting the oligonucleotides before the first region of the substrate is covalently coupled to the first region of the cover.
5. The method of claim 4 , wherein protecting the oligonucleotides comprises depositing a mask over the oligonucleotides.
6. The method of claim 5 , further comprising removing the mask after the first region of the substrate is covalently coupled to the first region of the cover.
7. The method of claim 2 , wherein the oligonucleotides are covalently coupled to the second region of the substrate using second moieties covalently coupled to the second region of the substrate.
8. The method of claim 2 , wherein the oligonucleotides comprise capture primers.
9. The method of claim 2 , wherein the oligonucleotides are coupled to the second region of the substrate after the first region of the substrate is coupled to the first region of the cover.
10. The method of claim 1 , wherein covalently coupling the first region of the substrate to the first region of the cover comprises selectively applying heat to the first region of the substrate or the first region of the cover.
11. The method of claim 10 , wherein the heat is applied using light.
12. The method of claim 11 , wherein the light includes an infrared or near-infrared wavelength.
13. The method of claim 1 , wherein covalently coupling the first region of the substrate to the first region of the cover comprises applying pressure to the first region of the substrate and the first region of the cover.
14. The method of claim 1 , wherein the intervening layer comprises the first moieties or the second moieties.
15. The method of claim 1 , wherein covalently coupling the first region of the cover to the intervening layer comprises a comprises a second azide-alkyne [3+2] cyclo-addition.
16. The method of claim 1 , wherein the first moieties are covalently coupled to the first region of the substrate via silane groups, carboxylate groups, or amidate groups.
17. The method of claim 1 , wherein the second moieties are covalently coupled to the second region of the substrate via silane groups, carboxylate groups, or amidate groups.
18. The method of claim 1 , wherein the cover comprises a different material than the substrate.
19. The method of claim 1 , wherein the cover comprises at least one material selected from the group consisting of: cyclic olefin polymer (COP), cyclic olefin copolymer (COC), glass, silicon, polypropylene (PP), photoresist, polyethylene terephthalate (PET), poly(N-(5-azidoacetamidylpentyl) acrylamide-co-acrylamide) (PAZAM), and polyethylene (PE).
20. The method of claim 1 , wherein the substrate comprises at least one material selected from the group consisting of: cyclic olefin polymer (COP), cyclic olefin copolymer (COC), glass, silicon, polypropylene (PP), photoresist, polyethylene terephthalate (PET), poly(N-(5-azidoacetamidylpentyl) acrylamide-co-acrylamide) (PAZAM), and polyethylene (PE).
21. The method of claim 1 , wherein the second region of the cover is separated from the second region of the substrate by between about 1 μm and about 1 cm.