METHOD FOR PREDICTING EFFICACY OF TREATMENT OF LUNG CANCER PATIENT USING IMMUNE CHECKPOINT INHIBITOR

1 . A method for predicting efficacy of treatment of a lung cancer patient using with an immune checkpoint inhibitor, the method comprising:

administering the immune checkpoint inhibitor to the lung cancer patient;

taking a biological sample from the lung cancer patient which has been administered with the immune checkpoint inhibitor;

isolating exosomes from the biological sample derived from the lung cancer patient; and

determining an expression level of a protein present in the exosomes by a mass spectrometry method,

wherein the protein is one or more proteins selected from the group of proteins shown in Table 1-1 to Table 1-6:

TABLE 1-1

Protein Name

Band 4.1 -like protein 2

Cullin-2

Protein Hikeshi

Hippocalcin-like protein 1

UTP–glucose-1 -phosphate uridylyltransferase

Attractin

Annexin A3

Rap1 GTPase-activating protein 2

Radixin

Annexin A5

ADP-ribosylation factor 5

L-lactate dehydrogenase B chain

Rho GTPase-activating protein 1

Drebrin

Serine/threonine-protein kinase PAK 2

Protein BRICK1

Integrin alpha-V

Protein kinase C and casein kinase substrate in neurons protein 2

Triokinase/FMN cyclase

Adhesion G-protein coupled receptor G1

Integrin beta-5

Isochorismatase domain-containing protein 1

Septin-2

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

Serine/threonine-protein kinase 38

Transient receptor potential cation channel subfamily M member 4

Cytoskeleton-associated protein 5

FAS-associated factor 1

Insulin receptor

Tropomyosin alpha-4 chain

Programmed cell death protein 6

Leukocyte elastase inhibitor

ADP-ribosylation factor 6

Complement component C9

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Actin, alpha cardiac muscle 1

Heat shock 70 kDa protein 4

14-3-3 protein theta

Phosphatidylinositol transfer protein beta isoform

Phosphoribosyltransferase domain-containing protein 1

Costars family protein ABRACL

Ubiquitin-conjugating enzyme E2 L5

Sodium/potassium-transporting ATPase subunit alpha-1

14-3-3 protein epsilon

TABLE 1-2

Major prion protein

F-actin-capping protein subunit alpha-2

HLA class I histocompatibility antigen, Cw-4 alpha chain

GTP-binding protein SAR1a

Actin-related protein ⅔ complex subunit 5

Glutathione reductase, mitochondrial

Guanine nucleotide-binding protein subunit alpha-11

Protein disulfide-isomerase A5

MARCKS-related protein

Septin-7

Hsc70-interacting protein

COP9 signalosome complex subunit 8

Vacuolar protein sorting-associated protein 37C

Acidic fibroblast growth factor intracellular-binding protein

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1

Ribosome maturation protein SBDS

Fructose-bisphosphate aldolase A

BH3-interacting domain death agonist

Charged multivesicular body protein 4b

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

cAMP-dependent protein kinase catalytic subunit alpha

Cytosolic non-specific dipeptidase

Dynamin-1

Neuroplastin

IST1 homolog

Sorting nexin-5

Mitochondrial import inner membrane translocase subunit Tim13

Exocyst complex component 4

Moesin

Serum albumin

Myristoylated alanine-rich C-kinase substrate

Sodium/potassium-transporting ATPase subunit beta-3

Vinculin

Actin-related protein ⅔ complex subunit 1B

Alcohol dehydrogenase class-3

Cysteine and glycine-rich protein 1

E3 ubiquitin-protein ligase TRIM23

Actin-related protein 3

Tetraspanin-15

Ras-related C3 botulinum toxin substrate 1

COP9 signalosome complex subunit 2

Myotrophin

Rho-related GTP-binding protein RhoF

COP9 signalosome complex subunit 3

Glutathione synthetase

DnaJ homolog subfamily C member 5

TABLE 1-3

Glutathione S-transferase omega-1

Glutathione S-transferase Mu 2

Ragulator complex protein LAMTOR2

Cytochrome b5 type B

Progressive ankylosis protein homolog

Septin-11

Rho-associated protein kinase 1

Ras-related C3 botulinum toxin substrate 3

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Inactive tyrosine-protein kinase transmembrane receptor ROR1

Abl interactor 2

Cell division control protein 42 homolog

Transgelin-2

Ras-related protein Rab-2A

Charged multivesicular body protein 6

ADP-ribosylation factor 3

Glyoxylate reductase/hydroxypyruvate reductase

Profilin-1

Ras-related protein Rab-11 B

Rab GDP dissociation inhibitor alpha

CD97 antigen

Programmed cell death protein 10

Antithrombin-III

F-actin-capping protein subunit beta

Actin-related protein ⅔ complex subunit 2

Twinfilin-2

Ras-related protein Rab-21

Vacuolar-sorting protein SNF8

Caspase-6

Calcineurin subunit B type 1

Extended synaptotagmin-2

Leukocyte surface antigen CD47

COP9signalosomecomplex subunit 1

26S proteasome non-ATPase regulatory subunit 7

Eukaryotic translation initiation factor 3 subunit C-like protein

Deoxycytidylate deaminase

CD276 antigen

Caspase-3

Leucine-rich repeat-containing protein 57

Aldose reductase

Diphosphoinositol polyphosphate phosphohydrolase 1

Coagulation factor XIII A chain

Hsp70-binding protein 1

Sorting nexin-4

Protein phosphatase 1L

Pleckstrin homology domain-containing family N member 1

TABLE 1-4

AP-2 complex subunit alpha-2

Trophoblast glycoprotein

Ras-related protein Rab-23

14-3-3 protein eta

Peroxiredoxin-6

14-3-3 protein beta/alpha

Copine-2

14-3-3 protein gamma

Superoxide dismutase [Cu-Zn]

Microtubule-associated protein RP/EB family member 1

Vasodilator-stimulated phosphoprotein

Phosphoglucomutase-1

Adenylyl cyclase-associated protein 1

Calpain small subunit 1

Dual specificity protein phosphatase 23

Dihydropteridine reductase

Ephrin-B1

Golgin subfamily A member 7

Xaa-Pro aminopeptidase 1

Actin-related protein ⅔ complex subunit 3

Sorting nexin-9

Pleckstrin homology domain-containing family O member 2

WD repeat-containing protein 44

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

COP9 signalosome complex subunit 6

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-10

Rab-like protein 3

Phosphoacetylglucosamine mutase

Sorting nexin-2

DDB1- and CUL4-associated factor 11

Ras GTPase-activating protein 1

Ribose-5-phosphate isomerase

SHC-transforming protein 1

Glutaminyl-peptide cyclotransferase-like protein

Ras-related protein Rab-5C

Purine nucleoside phosphorylase

Delta-aminolevulinic acid dehydratase

Ras-related protein Rab-5B

Abl interactor 1

Beta-2-glycoprotein 1

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Actin-related protein 2

Ras-related protein Rap-2a

Sorcin

Type I inositol 1,4.5-trisphosphate 5-phosphatase

Ras-related protein Rab-35

TABLE 1-5

Rho GTPase-activating protein 18

Ras-related protein Rab-1A

Deoxyribonuclease-1-like 1

Peptidyl-prolyl cis-trans isomerase A

Adenylate kinase isoenzyme 1

Equilibrative nucleoside transporter 1

GTPase NRas

Rho-related GTP-binding protein RhoG

Glycogen phosphorylase, brain form

UV excision repair protein RAD23 homolog B

Omega-amidase NIT2

Serine hydroxymethyltransferase, cytosolic

Sodium/potassium-transporting ATPase subunit beta-1

Endothelial cell-selective adhesion molecule

Isocitrate dehydrogenase [NADP] cytoplasmic

Peptidyl-prolyl cis-trans isomerase FKBP1A

Dystroglycan

Tumor susceptibility gene 101 protein

Peroxiredoxin-5, mitochondrial

Protein XRP2

Actin-related protein ⅔ complex subunit 4

Stathmin

NSFL1 cofactor p47

Folate receptor alpha

Aldo-keto reductase family 1 member A1

Ran-specific GTPase-activating protein

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Vacuolar protein sorting-associated protein VTA1 homolog

Dual specificity protein phosphatase 3

Membrane cofactor protein

Calcium-binding protein 39

Argininosuccinate lyase

Mitochondrial import receptor subunit TOM34

Protein numb homolog

SUMO-conjugating enzyme UBC9

EH domain-containing protein 3

Ras-related protein Rab-1B

Adenine phosphoribosyltransferase

Coronin-1A

Annexin A4

F-actin-capping protein subunit alpha-1

Protein phosphatase 1 regulatory subunit 7

Peflin

Protein/nucleic acid deglycase DJ-1

Ras-related protein Rab-10 Ras-related protein Rap-2b

TABLE 1-6

RNA transcription, translation and transport factor protein

Crk-like protein

Serpin B6

6-phosphogluconolactonase

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Lactadherin

Ras-related protein Ral-A

Protein transport protein Sec23A

Dihydropyrimidinase-related protein 2

NCK-interacting protein with SH3 domain

Vesicle-associated membrane protein 5

Protein phosphatase 1F

Nuclear receptor corepressor 2

Ubiquitin-conjugating enzyme E2 D3

WW domain-binding protein 2

POTE ankyrin domain family member I

Copine-1

Alpha-galactosidase A

Coronin-1B

ATP-dependent RNA helicase DDX1

Ubiquitin-conjugating enzyme E2 K

Syntaxin-binding protein 1

WD repeat-containing protein 37

MOB kinase activator 1B

Nodal modulator 3

Nucleoside diphosphate kinase B

Prostaglandin reductase 1

Phosphoglycerate mutase 2

TLD domain-containing protein 1

Calcium-binding protein 39-like

Glutathione S-transferase LANCL1

Prefoldin subunit 6

Cytosolic phospholipase A2

14-3-3 protein zeta/delta

.

2 . The method according to claim 1 ,

wherein the protein is one or more membrane proteins selected from the group of proteins shown in Table 2:

TABLE 2

Protein Name

Band 4.1-like protein 2

Attractin

Rho GTPase-activating protein 1

Integrin alpha-V

Adhesion G-protein coupled receptor G1

Integrin beta-5

Transient receptor potential cation channel subfamily M member 4

Insulin receptor

Sodium/potassium-transporting ATPase subunit alpha-1

Major prion protein

Fructose-bisphosphate aldolase A

Neuroplastin

Sodium/potassium-transporting ATPase subunit beta-3

Tetraspanin-15

DnaJ homolog subfamily C member 5

Cytochrome b5 type B

Progressive ankylosis protein homolog

Inactive tyrosine-protein kinase transmembrane receptor ROR1

CD97 antigen

Extended synaptotagmin-2

Leukocyte surface antigen CD47

CD276 antigen

Protein phosphatase 1L

Trophoblast glycoprotein

Ephrin-B1

Equilibrative nucleoside transporter 1

Sodium/potassium-transporting ATPase subunit beta-1

Endothelial cell-selective adhesion molecule

Peptidyl-prolyl cis-trans isomerase FKBP1A

Dystroglycan

Folate receptor alpha

Membrane cofactor protein

NCK-interacting protein with SH3 domain

Vesicle-associated membrane protein 5

Syntaxin-binding protein 1

Nodal modulator 3

.

3 . The method according to claim 1 ,

wherein the protein is one or more proteins selected from the group of proteins shown in Table 3:

TABLE 3

Protein Name

Integrin beta-5

Sorting nexin-9

Phosphoacetylglucosamine mutase

Stathm in

Ran-specific GTPase-activating protein

EH domain-containing protein 3

Adenine phosphoribosyltransferase

Protein phosphatase 1 regulatory subunit 7

Nuclear receptor corepressor 2

TLD domain-containing protein 1

Glutathione S-transferase LANCL 1

.

4 . The method according to claim 1 ,

wherein the immune checkpoint inhibitor is an anti-PD-1 antibody or an anti-PD-L1 antibody.

5 . The method according to claim 1 ,

wherein the immune checkpoint inhibitor is one or more selected from the group consisting of nivolumab, pembrolizumab, and atezolizumab.

6 . The method according to claim 1 , comprising:

isolating the exosomes from the biological sample derived from the lung cancer patient with a mixture of magnetic particles bound to an anti-CD9 antibody, an anti-CD81 antibody, or an anti-CD63 antibody is used.