IP Library Granted Patent US 12,364,266
Granted Patent B2
US 12,364,266 · App. 17/764,498 · Granted Jul 22, 2025

Pseudomonas isolates and uses thereof

Inventors: David Michael Barnhart (Apex, NC); William Nathan Cude (Durham, NC); Kate Brandon Sutton (Raleigh, NC); Timothy Liburn (Durham, NC); Jonathan Pham (Durham, NC); Gregory Stephen Maloney (Creedmoor, NC); Jeffrey Layman (Durham, NC)
Assignee: NOVOZYMES BIOAG A/S
A01N63/20C12N1/205C12R2001/38
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Quick Facts
Patent No.
US 12,364,266
App. No.
17/764,498
Granted
Jul 22, 2025
Kind
B2
Abstract

The present disclosure provides isolated Pseudomonas koreensis strains useful for enhancing crop yield, as well as inoculant compositions comprising one or more of the isolated strains, seeds that have been treated with one or more of the isolated strains, and methods of using the isolated strains to enhance root growth, nutrient uptake, chlorophyll content, etc.

Claims (20)

1. A non-naturally occurring inoculant composition comprising Pseudomonas koreensis NRRL B-67883 in an agriculturally acceptable carrier.

2. The composition of claim 1 , said composition comprising at least 1×104 colony-forming units (CFU) of Pseudomonas koreensis NRRL B-67883 per gram of said composition and/or at least 1×104 colony-forming units (CFU) of Pseudomonas koreensis NRRL B-67883 per milliliter of said composition.

3. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more maltodextrins.

4. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more disaccharides.

5. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more sugar alcohols.

6. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more maltodextrins and one or more disaccharides.

7. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more maltodextrins and one or more sugar alcohols.

8. The composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more maltodextrins, one or more disaccharides, and one or more sugar alcohols.

9. The composition of claim 1 , wherein said agriculturally acceptable carrier is a powder or granule.

10. The composition of claim 1 , wherein said agriculturally acceptable carrier is an aqueous liquid.

11. The composition of claim 1 , wherein said agriculturally acceptable carrier is a non-aqueous liquid.

12. A method comprising introducing the composition of claim 1 into a plant growth medium.

13. A method comprising contacting a plant seed with the composition of claim 1 .

14. A method comprising contacting a plant with the composition of claim 1 .

15. A plant seed that is at least partially coated with the composition of claim 1 .

16. A method comprising inoculating a culture medium with Pseudomonas koreensis NRRL B-67883 and incubating the inoculated culture medium at a temperature of about 4° C. to about 37° C., thereby producing an incubation product that comprises Pseudomonas koreensis NRRL B-67883.

17. A method comprising introducing one or more stabilizers into the incubation product of claim 16 , said one or more stabilizers comprising one or more maltodextrins and/or one or more disaccharides and/or one or more sugar alcohols.

18. A method comprising isolating Pseudomonas koreensis NRRL B-67883 from the incubation product of claim 16 .

19. A kit comprising the incubation product of claim 16 and one or more stabilizers, said one or more stabilizers comprising one or more maltodextrins and/or one or more disaccharides and/or one or more sugar alcohols.

20. A method comprising contacting a plant or plant part with the incubation product of claim 16 .

Assignments (2)
CHANGE OF NAME AND ADDRESS Recorded Oct 7, 2024
From: NOVOZYMES BIOAG A/S
To: NOVONESIS PLANT BIOSOLUTIONS A/S
Reel/Frame 070166/0088 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 28, 2022
From: BARNHART, DAVID MICHAEL; CUDE, WILLIAM NATHAN; SUTTON, KATE BRANDON; LIBURN, TIMOTHY; PHAM, JONATHAN; MALONEY, GREGORY STEPHEN; LAYMAN, JEFFREY
To: NOVOZYMES BIOAG A/S
Reel/Frame 059417/0608 →
Continuity (2)
Provisional Application 62937929 · Nov 20, 2019
Related Publication 20220346383A1 · Nov 3, 2022
References Cited (24)
US 5484464A · Gleddie · 1996 [cited by applicant]
US 5586411A · Gleddie · 1996 [cited by applicant]
US 5695541A · Kosanke · 1997 [cited by applicant]
US 5804208A · Andersch · 1998 [cited by applicant]
US 5916029A · Smith · 1999 [cited by applicant]
US 6569425B2 · Drahos · 2003 [cited by applicant]
US 6808917B1 · Johnson · 2004 [cited by applicant]
US 6824772B2 · Drahos · 2004 [cited by applicant]
US 7429477B2 · Johnson · 2008 [cited by applicant]
US 8148138B2 · Johnson · 2012 [cited by applicant]
US 8278247B2 · Hnatowich · 2012 [cited by applicant]
US 8445256B2 · Woods · 2013 [cited by applicant]
US 8883679B2 · Woods · 2014 [cited by applicant]
US 8921089B2 · Kang · 2014 [cited by applicant]
US 8999698B2 · Kang · 2015 [cited by applicant]
US 9017442B2 · Johnson · 2015 [cited by applicant]
US 9101088B2 · Hnatowich · 2015 [cited by applicant]
US 9234251B2 · Snyder · 2016 [cited by applicant]
US 9340464B2 · Hnatowich · 2016 [cited by applicant]
CN 105586303A · 2016 [cited by applicant]
KR 101865810B1 · 2018 [cited by applicant]
RU 2694565C1 · 2019 [cited by applicant]
Anonymous, 2018, Genbank No. MH542340.1. [cited by applicant]
Babu et al, 2015, Journal of environmental management, vol. 151, pp. 160-166. [cited by applicant]