Granted Patent B2

US 12,569,175 · App. 17/765,266 · Granted Mar 10, 2026

Inventors: Frédéric Bebien (Jardin, FR); Stéphanie Pigeot Remy (Sainte Colombe, FR)

Assignee: AHLSTROM OYJ

A61B5/150755B01D39/163B01D39/2024G01N33/491B01D2239/0421B01D2239/086B01D2239/1208B01D2239/1216

Abstract

Claims (33)

1 . Blood components collection and separation media ( 1 ) comprising: a substrate ( 3 ) configured to be wetted by a whole blood sample ( 5 ), the substrate ( 3 ) having a maximal flow pore size comprised between 8 μm and 35 μm, such maximal flow pore size enabling the retention of at least red cells ( 51 ) on the surface of the substrate ( 3 ), characterized in that the blood components collection and separation media ( 1 ) further comprises boundary walls ( 7 ) extending at least through a whole depth (d) of the substrate ( 3 ) which is a fibrous web and which forms a pattern ( 9 ) into said substrate ( 3 ), said boundary walls ( 7 ) being made of a hydrophobic resin, and in that the pattern ( 9 ) within the substrate ( 3 ) and formed by the boundary walls ( 7 ) comprises:

a collection zone ( 91 ) aimed at receiving the whole blood sample ( 5 );

at least one storage zone ( 93 ) aimed at collecting at least one component of the whole blood sample ( 5 ) after its separation through the substrate ( 3 ); and

at least one channel ( 95 ) connecting the collection zone ( 91 ) to the at least one storage zone ( 93 ), the at least one channel ( 95 ) forming a bottleneck between the collection zone ( 91 ) and the at least one storage zone ( 93 ).

2 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the substrate ( 3 ) has a mean flow pore size comprised between 2.5 μm and 5 μm.

3 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the hydrophobic resin forming the boundary walls ( 7 ) is chosen among photo-curable resins, UV-curable resins, or thermosetting resins.

4 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the hydrophobic resin is chosen among fluorinated resins, modified fluorinated resins, latexes, glycol ether acrylates, acrylate esters, or a combination thereof.

5 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the collection zone ( 91 ) of the pattern ( 9 ) has a substantially parallelogram shape; and

wherein the at least one storage zone ( 93 ) is disposed at an angle ( 97 ) of the substantially parallelogram shape of the collection zone ( 91 ); and

wherein the pattern ( 9 ) comprises as many storage zones ( 93 ) as the collection zone ( 91 ) has channels ( 95 ).

6 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the at least one storage zone ( 93 ) has a substantially circular shape.

7 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the at least one channel ( 95 ) has a length ( 1 ) comprised between 1 mm and 5 mm and a width (w) comprised between 1 mm and 3 mm.

8 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the fibrous web comprises:

first fibers, said first fibers being chosen among glass micro-fibers or synthetic micro-fibers;

second fibers, said second fibers being chosen among fibrillated fibers; and

a hydrophilic binder.

9 . Blood components collection and separation media ( 1 ) according to claim 8 , wherein the hydrophilic binder is at least one of latex binders, polyvinyl alcohol binders, styrene-butadiene binders, vinyl-acetate binders, polysaccharide binders or protein binders.

10 . Blood components collection and separation media ( 1 ) according to claim 8 , wherein the fibrous web comprises between 5 and 7% by weight of the hydrophilic binder.

11 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the boundary walls ( 7 ) forming the pattern ( 9 ) exceed one surface of the substrate ( 3 ).

12 . Blood components collection and separation media ( 1 ) according to claim 1 , wherein the collection zone ( 91 ) further contains a salt capable of achieving at least partial crenation of red cells ( 51 ), the salt being chosen from the group of alkali metal and alkaline earth metal halogenides and sulfates, and hydrochlorides of organic bases.

13 . Blood components collection and separation device ( 10 ), aimed at separating and collecting at least one component of a whole blood sample ( 5 ), wherein the blood components collection and separation device ( 10 ) comprises at least the blood components collection and separation media ( 1 ) according to claim 1 and a frame ( 11 ) surrounding the blood components collection and separation media ( 1 ).

14 . Blood components separation and extraction process ( 100 ), wherein the process ( 100 ) implements a blood components collection and separation media ( 1 ) according to claim 1 and the process ( 100 ) comprises the following steps:

depositing (S 1 ) of a whole blood sample ( 5 ) in the collection zone ( 91 );

absorbing (S 3 ) of the whole blood sample ( 5 ) onto the substrate ( 3 );

retaining (S 5 ) of the red cells ( 51 ), the platelets ( 52 ) and the white cells ( 53 ) on the substrate ( 3 ) in the collection zone ( 91 );

diffusing (S 7 ) of at least one component through the substrate ( 3 ) to the at least one storage zone ( 93 ); and

extracting (S 9 ) at least one target component stored in the substrate ( 3 ) of the blood components collection and separation media ( 1 ).

15 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the at least one target component is stored in the at least one storage zone ( 93 ) and wherein the extraction step (S 9 ) of said at least one target component is performed by punching at least one part of said at least one storage zone ( 93 ) or by eluting said at least one target component from the at least one storage zone ( 93 ).

16 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the at least one target component is stored in the collection zone ( 91 ) and wherein the extraction step (S 9 ) of said at least one target component is performed by punching at least one part of said collection zone ( 91 ) or by eluting said at least one target component from the collection zone ( 91 ).

17 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the whole blood sample ( 5 ) deposited in the collection zone ( 91 ) has a volume comprised between 50 μL and 500 μL.

18 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the target component aimed at being extracted from the whole blood sample ( 5 ) is plasma ( 55 ).

19 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the target component aimed at being extracted from the whole blood sample ( 5 ) is DNA, said DNA being stored in the white cells ( 53 ).

20 . Blood components separation and extraction process ( 100 ) according to claim 14 , wherein the target component aimed at being extracted from the whole blood sample ( 5 ) is lipids, said lipids being stored in the red cells ( 51 ).

Assignments (2)

Continuity (1)

References Cited (29)