IP Library › Granted Patent US 12,577,536
Granted Patent B2
US 12,577,536 · App. 17/765,358 · Granted Mar 17, 2026

Method for isolating ureteric bud tip cells

Inventors: Kenji Osafune (Kyoto, JP); Shinichi Mae (Kyoto, JP); Makoto Ryosaka (Kyoto, JP)
Assignee: KYOTO UNIVERSITY
C12N5/0684G01N33/56966C12N2501/11C12N2501/113C12N2501/119C12N2501/13C12N2501/155C12N2501/16C12N2501/415C12N2501/999C12N2506/45
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Quick Facts
Patent No.
US 12,577,536
App. No.
17/765,358
Granted
Mar 17, 2026
Kind
B2
Abstract

Provided is a method for isolating a ureteric bud tip cell from cells, a tissue, or an organoid comprising the ureteric bud tip cell, comprising the following steps of contacting the cells, tissue, or organoid comprising the ureteric bud tip cell with a very low density lipoprotein receptor (VLDL-R) binding agent, and isolating the ureteric bud tip cell using the binding agent as an indicator.

Claims (17)

1 . A method for isolating a ureteric bud tip cell from cells, a tissue, or an organoid comprising the ureteric bud tip cell, comprising the following steps of:

(1-1) contacting the cells, tissue, or organoid comprising the ureteric bud tip cell with a labelled very low density lipoprotein (VLDL), and

(1-2) isolating the ureteric bud tip cell using the labelled VLDL as an indicator,

wherein the cells, tissue, or organoid comprising the ureteric bud tip cell is differentiated from a human induced pluripotent stem cell.

2 . The method according to claim 1 , wherein the cells, tissue, or organoid comprising the ureteric bud tip cell is a ureteric bud-like organoid.

3 . A method for producing a ureteric bud tip cell colony, comprising:

isolating a ureteric bud tip cell by the method according to claim 1 , and the step of

(2) culturing the ureteric bud tip cell in a medium comprising glial cell line-derived neurotrophic factor, a fibroblast growth factor, a retinoic acid receptor agonist, a GSK3β inhibitor, and a Yes-associated protein (YAP) activity inhibitor.

4 . The method according to claim 3 , wherein in step (2), the medium further comprises a TGFβ signal inhibitor.

5 . The method according to claim 3 , comprising a step of passaging the obtained ureteric bud tip cell colony after step (2).

6 . A method for producing a ureteric bud-like organoid, comprising:

obtaining a ureteric bud tip cell colony by the method according to claim 3 , and the step of

(3) culturing the ureteric bud tip cell colony in a medium comprising a Wnt signaling activator, a BMP inhibitor, a fibroblast growth factor, a retinoic acid receptor agonist, and glial cell line-derived neurotrophic factor to reconstitute the ureteric bud-like organoid.

7 . The method according to claim 6 , wherein in step (3), the medium further comprises EGF and FGF1.

8 . A method for producing a collecting duct progenitor-like organoid, comprising:

obtaining a ureteric bud-like organoid by the method according to claim 6 , and the step of

(4) culturing the ureteric bud-like organoid in a medium comprising a Wnt signal inhibitor and a TGFβ signal inhibitor.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 30, 2022
From: OSAFUNE, KENJI; MAE, SHINICHI; RYOSAKA, MAKOTO
To: KYOTO UNIVERSITY
Reel/Frame 059447/0648 →
Priority Claims (1)
JP 2019-181593 · Oct 1, 2019 · national
Continuity (1)
Related Publication 20220380732A1 · Dec 1, 2022
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