NON-FREEZING REFRIGERATED STORAGE LIQUID FOR STEM CELLS

1 . A refrigerated storage liquid for storing human or animal stem cells or embryos in a non-frozen state, comprising:

potassium ion species in a range of 30 to 80 mmoL/L;

sodium ion species in a range of 30 to 80 mmoL/L, an ion-based molar ratio of the sodium ion species to the potassium ion species being in a range of 0.5 to 1.3;

Trolox or analog thereof at a concentration of 0.5 to 8 mM;

adenine or a salt or derivative thereof at a concentration of 0.1 to 4.2 mM;

all of or all but one, two or three kinds of essential amino acids, a total content of which being 50 to 200 mg/L; and

non-essential amino acids, a total content of which being 50 to 200 mg/L.

2 . A refrigerated storage liquid for storing human or animal stem cells or embryos in a non-frozen state, comprising:

(i) lactobionic acid or salt thereof in terms of lactone in a range of 30 to 100 mmoL/L;

(ii) raffinose hydrate in a range of 10 to 30 mmoL/L;

(iii) alloprinol in a range of 0.3 to 1 mmoL/L;

(iv) total glutathione in a range of 1 to 3 mmoL/L;

(v) adenosine in a range of 2 to 10 mmoL/L;

(vi) lipoic acid in a range of 0.1 to 0.5 µmoL/L;

(vii) sodium pyruvate in a range of 0.1 to 0.7 mmoL/L;

(viii) glucose in a range of 1 to 5 mmoL/L;

(ix) ascorbic acid in a range of 0.03 to 0.3 mmoL/L and vitamin E or water-soluble analog/derivative thereof in a range of 0.5 to 8 mM;

(x) adenine, or salt or derivative thereof in a range of 0.1 to 4.2 mM;

(xi) vitamins or water-soluble analog/derivatives thereof, including folic acid, nicotine amide, riboflavin, B12, choline, inositol, pantothenic acid, pyridoxal phosphate and thiamine;

(xii) essential amino acids 50 to 200 mg/L in total;

(xiii) non-essential amino acids, in a range of 100 to 500 mg/L in total;

(xiv) potassium ion species in a range of 30 to 80 mmoL/L; and

(xv) sodium ion species in a range of 20 to 90 mmoL/L.

3 . The refrigerated storage liquid according to claim 1 , further comprising:

20 to 50 g/L of hydroxyethyl starch,

5 to 50 g/L of polyvinyl alcohol, and/or

30 to 90 mmoL/L of mannitol.

4 . The refrigerated storage liquid according to claim 1 , further comprising:

L-alanyl-L-glutamine or dipeptide substitute, or a salt thereof, at a concentration of 2 to 5 mM.

5 . A refrigerated storage method comprising:

suspending or immersing human or animal iPS cells or artificial stem cells, stem cells, organoids obtained therefrom, or embryos in the refrigerated storage liquid according to claim 1 and

storing the cells in a suspended or immersed state, at 2 to 8° C. for 1 to 10 days.

6 . The refrigerated storage liquid according to claim 2 , further comprising:

20 to 50 g/L of hydroxyethyl starch,

5 to 50 g/L of polyvinyl alcohol, and/or

30 to 90 mmoL/L of mannitol.

7 . The refrigerated storage liquid according to claim 2 , further comprising:

L-alanyl-L-glutamine or dipeptide substitute, or a salt thereof, at a concentration of 2 to 5 mM.

8 . A refrigerated storage method comprising:

suspending or immersing human or animal iPS cells or artificial stem cells, stem cells, organoids obtained therefrom, or embryos in the refrigerated storage liquid according to claim 2 ; and

storing the cells in a suspended or immersed state, at 2 to 8° C. for 1 to 10 days.