Composite demineralized bone matrix composition containing bone mineral component and method for producing same
A method for producing a composite demineralized bone matrix composition using a one-step process is described. The composite demineralized bone matrix composition is produced from the biologically-derived bone. In addition, the composite demineralized bone matrix composition contains bone minerals according to the original composition proportion in the bone and may provide a bone mineral content condition that is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.
1 . A method of preparing a composite demineralized bone matrix (DBM) composition using a one-step process, comprising:
a demineralization step of demineralizing bone tissue with an acidic solution;
a bone mineral precipitation step of precipitating bone mineral by neutralizing the demineralized solution with an alkaline solution;
a bone morphogenetic protein-2 (BMP-2) extraction step of extracting BMP-2 by centrifuging the solution in which the precipitation is completed and adding an acidic solution to a pellet; and
a composite DBM composition preparation step of precipitating the DBM, the bone mineral, and the BMP-2 by neutralizing the BMP-2-extracted solution with an alkaline solution.
2 . The method of claim 1 , wherein the bone tissue is cortical bone, and has an average particle diameter of 20 to 800 μm.
3 . The method of claim 1 , wherein the demineralization step is performed by demineralization with 10 to 50 ml of a 0.5 to 1.5N hydrochloric solution per 1 g of the bone tissue.
4 . The method of claim 1 , wherein the bone mineral precipitation step is performed by neutralization with a 0.5 to 4.0M sodium hydroxide (NaOH) solution.
5 . The method of claim 1 , wherein the BMP-2 extraction step is performed by adding acetic acid to the pellet.
6 . The method of claim 1 , wherein the composite DBM composition preparation step is to precipitate DBM, the bone mineral and BMP-2 by neutralization with a 0.5 to 4.0M sodium hydroxide (NaOH) solution.
7 . The method of claim 1 , further comprises:
drying the resulting precipitate, after the composite DBM composition preparation step.