Devices containing cellular membrane and uses thereof
The present disclosure relates to devices, e.g, extracorporeal blood purification (EBP) devices, which comprise a cartridge or sorbent comprising a cellular membrane derived from a cell. The present disclosure also relates methods for removing or reducing a substance, e.g, an undesirable substance, from a fluid, e.g, a subject's blood, using the devices or EBP devices.
1 . A device for extracorporeal detoxification, which comprises a cartridge or sorbent comprising a cellular membrane derived from a cell, and
wherein said cartridge or sorbent has a ratio between a surface area of said cellular membrane and a volume of said cartridge or sorbent that ranges from about 1×10 4 m −1 to about 3×10 8 m −1 .
2 . The device of claim 1 , wherein the cartridge or sorbent has a ratio between the surface area of the cellular membrane and the volume of the cartridge or sorbent that ranges from about 1×10 5 m −1 to about 3×10 8 m −1 .
3 . The device of claim 1 , wherein the cartridge or sorbent comprises nanoparticles comprising the cellular membrane, nanostructures comprising the cellular membrane, plenary surfaces comprising the cellular membrane, or a combination thereof.
4 . The device of claim 1 , wherein the cartridge or sorbent comprises nanoparticles comprising the cellular membrane.
5 . The device of claim 3 , wherein the cellular membrane of the nanoparticle comprises a plasma membrane or an intracellular membrane.
6 . The device of claim 3 , wherein the cellular membrane of the nanoparticle is derived from a multicellular organism.
7 . The device of claim 3 , wherein the nanoparticle further comprises a releasable cargo.
8 . The device of claim 3 , wherein the nanoparticle has a diameter from about 10 nm to about 10 μm.
9 . The device of claim 3 , wherein the nanoparticle substantially lacks constituents of the cell from which the cellular membrane is derived.
10 . The device of claim 3 , wherein the nanoparticle substantially maintains natural structural integrity or activity of the cellular membrane, or the constituents of the cellular membrane.
11 . The device of claim 3 , the nanoparticle is biocompatible or biodegradable.
12 . The device of claim 3 , wherein the inner core of the nanoparticle comprises PLGA and the outer surface comprises a plasma membrane derived from a red blood cell.
13 . The device of claim 3 , wherein the nanoparticle substantially lacks immunogenicity to a species or subject from which the cellular membrane is derived.
14 . The device of claim 3 , wherein the outer surface comprises a naturally occurring cellular membrane and further comprises a modified cellular membrane and/or a synthetic membrane.
15 . The device of claim 1 , wherein the cartridge or sorbent comprises nanostructures comprising the cellular membrane.
16 . The device of claim 1 , wherein the cartridge or sorbent comprises nanofibers comprising the cellular membrane.
17 . The device of claim 1 , wherein the cartridge or sorbent comprises at least 2 or all 3 of the following:
1) nanoparticles comprising the cellular membrane;
2) nanostructures comprising the cellular membrane;
3) plenary surfaces comprising the cellular membrane.
18 . The device of claim 1 , wherein the cellular membrane is derived from a blood cell, a tumor cell, a cancer cell, an immune cell, a stem cell, an endothelial cell, a neuronal cell, an exosome, a secretory vesicle and/or a synaptic vesicle.
19 . The device of claim 1 , which has a length that ranges from 0.01 m to 1 m (meter).
20 . The device of claim 1 , which has a cross-section surface area that ranges from 1×10 −3 m 2 (square meter) to about 0.01 m 2 (square meter).
21 . The device of claim 1 , which is an extracorporeal blood purification (EBP) device.
22 . The device of claim 1 , wherein the cellular membranes are derived from different types of cells.
23 . A method for removing or reducing a substance from a liquid which method comprises, ex vivo, contacting a liquid with the device of claim 1 .