Granted Patent
B2
US 12,644,154 · App. 17/812,678 · Granted Jun 2, 2026
Determining WT-1-specific T cells and WT-1 specific T cell receptors (TCRS)
Inventors:
Marcos E. Milla (Seattle, WA); Mark Klinger (Seattle, WA); Peter J.R. Ebert (Seattle, WA); Timothy L. Hayes (Seattle, WA); Edward J. Osborne (Seattle, WA); Joyce K. Hu (Seattle, WA)
Assignee:
Adaptive Biotechnologies Corporation
C12Q1/6881A61K40/11A61K40/32A61K40/4243C07K14/4748C07K14/7051C07K16/30A61K2039/804C07K2317/73C12Q2600/106C12Q2600/158C12Q2600/16
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Abstract
The invention is directed to methods for determining antigen-specific T cells generally and to T cell receptors which bind an epitope of the Wilms' tumor antigen-1 (WT1) protein specifically. The disclosure also provides polynucleotides encoding the TCRs, engineered cells exogenously expressing the TCRs, and methods of making and using the TCRs and/or cells expressing the TCRs.
Claims (8)
1 . One or more nucleic acids encoding a recombinant T cell receptor (TCR) that binds Wilms' tumor antigen-1 (WT1), wherein the recombinant TCR comprises an alpha chain and a beta chain, and wherein the beta chain comprises a CDR3 sequence comprising the amino acid sequence set forth in SEQ ID NO: 6, 14, 22, 30, 38, or 46.
2 . The one or more nucleic acids of claim 1 , wherein the beta chain comprises a CDR3 sequence comprising the amino acid sequence set forth in SEQ ID NO: 22.
3 . The one or more nucleic acids of claim 1 , wherein the alpha chain comprises a CDR3 sequence comprising the amino acid sequence set forth in SEQ ID NO: 19.
4 . The one or more nucleic acids of claim 2 , wherein the alpha chain comprises a CDR3 sequence comprising the amino acid sequence set forth in SEQ ID NO: 19.
5 . A recombinant host cell comprising the one or more nucleic acids of claim 1 .
6 . A recombinant host cell comprising the one or more nucleic acids of claim 2 .
7 . A recombinant host cell comprising the one or more nucleic acids of claim 3 .
8 . A recombinant host cell comprising the one or more nucleic acids of claim 4 .
Assignments (2)
SECURITY INTEREST
Recorded Sep 15, 2022
From: ADAPTIVE BIOTECHNOLOGIES CORPORATION
To: ORBIMED ROYALTY & CREDIT OPPORTUNITIES IV, LP
Reel/Frame 061449/0689 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 11, 2022
From: MILLA, MARCOS E.; KLINGER, MARK; EBERT, PETER J.R.; HAYES, TIMOTHY LEE; OSBORNE, EDWARD J.; HU, JOYCE K.
To: ADAPTIVE BIOTECHNOLOGIES CORPORATION
Reel/Frame 060783/0393 →
Continuity (6)
Provisional Application
62629496
· Feb 12, 2018
Provisional Application
62519088
· Jun 13, 2017
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