Antigen-specific helper T-cell receptor genes
The present invention pertains to polynucleotides that encode CDR3 in TCR-[alpha] and TCR-[beta] chain genes of CD4 + helper T-cells that are specific to WT1 helper peptides having an amino acid sequence represented by SEQ ID NO: 123. The present invention further pertains to the peptides encoded by said polynucleotides. The present invention further pertains to CD4 + T cells into which TCR genes that contain said polynucleotides have been introduced, the induction of WT1-specific cytotoxic T-lymphocytes (CTLs) using the CD4 + T-cells, the treatment of cancer, etc.
1. A method for the treatment of a WT1-expressing cancer in a subject, comprising introducing a CD4 + helper T-cell into the subject,
wherein the CD4 + helper T cell is obtained by introducing a TCR gene into a CD4 + T cell, wherein
(i) the TCR gene comprises a pair of polynucleotides chosen from:
(a) SEQ ID NO: 1 and SEQ ID NO: 2;
(b) SEQ ID NO: 3 and SEQ ID NO: 4;
(c) SEQ ID NO: 5 and SEQ ID NO: 6;
(d) SEQ ID NO: 3 and SEQ ID NO: 7;
(e) SEQ ID NO: 8 and SEQ ID NO: 9;
(f) SEQ ID NO: 10 and SEQ ID NO: 12;
(g) SEQ ID NO: 11 and SEQ ID NO: 12;
(h) SEQ ID NO: 13 and SEQ ID NO: 15,
(i) SEQ ID NO: 14 and SEQ ID NO: 15;
(j) SEQ ID NO: 16 and SEQ ID NO: 17;
(k) SEQ ID NO: 18 and SEQ ID NO: 19;
(l) SEQ ID NO: 20 and SEQ ID NO: 21;
(m) SEQ ID NO: 22 and SEQ ID NO: 24;
(n) SEQ ID NO: 23 and SEQ ID NO: 24;
(o) SEQ ID NO: 25 and SEQ ID NO: 26;
(p) SEQ ID NO: 27 and SEQ ID NO: 4;
(q) SEQ ID NO: 28 and SEQ ID NO: 29;
(r) SEQ ID NO: 30 and SEQ ID NO: 32;
(s) SEQ ID NO: 31 and SEQ ID NO: 32;
(t) SEQ ID NO: 33 and SEQ ID NO: 34;
(u) SEQ ID NO: 35 and SEQ ID NO: 36;
(v) SEQ ID NO: 37 and SEQ ID NO: 38;
(w) SEQ ID NO: 39 and SEQ ID NO: 40;
(x) SEQ ID NO: 41 and SEQ ID NO: 42;
(y) SEQ ID NO: 43 and SEQ ID NO: 44;
(z) SEQ ID NO: 45 and SEQ ID NO: 46;
(aa) SEQ ID NO: 47 and SEQ ID NO: 48;
(bb) SEQ ID NO: 49 and SEQ ID NO: 50;
(cc) SEQ ID NO: 51 and SEQ ID NO: 52;
(dd) SEQ ID NO: 53 and SEQ ID NO: 54;
(ee) SEQ ID NO: 55 and SEQ ID NO: 57;
(ff) SEQ ID NO: 56 and SEQ ID NO: 57; and
(gg) SEQ ID NO: 58 and SEQ ID NO: 59; or
(ii) the TCR gene comprises a pair of polynucleotides that are complementary to or degenerate of any of the pairs of polynucleotides listed in (i).