Integrated solid-state rapid thermo-cycling system
View Patent ↗Techniques, systems, and devices are disclosed for implementing a portable lab system for PCR testing. An example method for operating an integrated thermal cycling system includes depositing samples into the integrated thermal cycling system that includes a thermal cycling device and an electronic interface. The thermal cycling device includes multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element. The multiple wells are positioned within the substrate that includes a thermally conductive ground positioned between adjacent wells. Supplying power to the integrated thermal cycling system, via the electronic interface, allows the multiple wells to exchange heat with the substrate and for each well to operate independently from other wells.
1. A method of operating an integrated thermal cycling system, comprising:
depositing samples into the integrated thermal cycling system, wherein the integrated thermal cycling system comprises a thermal cycling device and an electronic interface, wherein the thermal cycling device comprises: multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element to control operation of the TEC element, wherein the multiple wells are positioned within the substrate, and wherein the substrate comprises a thermally conductive ground positioned between adjacent wells;
coupling the integrated thermal cycling system to a computing device via the electronic interface;
supplying power to the integrated thermal cycling system, via the electronic interface, to allow the multiple wells to exchange heat with the substrate and to allow each well of the multiple wells to operate independently from one another;
wherein supplying of the power to the integrated circuit comprises: transferring energy from a first well to a second well of the multiple wells when current flows through the TEC element in a first direction, and from the second well to the first well of the multiple wells when current flows through the TEC element in a second direction; and
collecting data from the controller of the integrated thermal cycling system via a data line.
2. The method of claim 1 , comprising: connecting the computing device to a network, and transmitting, via a network interface on the computing device, the data collected by the integrated thermal cycling system to other computing devices on the network.
3. The method of claim 1 , comprising: displaying, via a user interface on the computing device, the data collected by the integrated thermal cycling system.
4. The method of claim 1 , wherein the samples in at least one well of the multiple wells have a different thermal set point and a different cycle duration compared to samples in at least another one of the multiple wells.
5. The method of claim 1 , wherein the electronic interface is one of a USB interface or a FireWire interface.
6. The method of claim 1 , wherein the computing device is one of a personal computer or a mobile device.
7. The method of claim 1 , wherein the thermally conductive ground is a thermal ground lattice, line, trace, or plane.
8. The method of claim 1 , wherein the thermally conductive ground has a grid geometry between the adjacent wells.
9. The method of claim 1 , wherein the thermally conductive ground has a rectilinear shape outside of the multiple wells.
10. The method of claim 1 , wherein the thermally conductive ground forms a concentric ring outside or inside of the multiple wells.
11. The method of claim 1 , further comprising: operating the integrated thermal cycling system at a cycle time of approximately 5 seconds and a temperature from 95° to 50° Celsius.
12. The method of claim 1 , wherein each well of the multiple wells is insulated from any other well of the multiple wells, and each well of the multiple wells is in thermal contact with at least a portion of the TEC element and with the thermally conductive ground.
13. The method of claim 1 , further comprising providing a fan to maintain a temperature of the integrated thermal cycling system at a range of temperatures.
14. The method of claim 1 , wherein depositing the samples into the integrated thermal cycling system includes depositing a first set of samples into a first well of the multiple wells and a second set of samples into a second well of the multiple wells, and wherein the method comprises operating the first well of the multiple wells based on a first thermocycling temperature, and operating the second well of the multiple wells independently from the first well of the multiple wells based on a second thermocycling temperature.
15. The method of claim 14 , wherein operating the first and the second wells of the multiple wells are carried out concurrently.
16. The method of claim 1 , further comprising calibrating at least one of the multiple wells independently from at least another one of the multiple wells.