Electrical sensing, tracking, and actuation of droplets
Devices, techniques, and processes are disclosed that use electrical impedance to detect of the presence and contents of droplets including cells, nucleic acids, proteins, or solute concentrations in an array of retrievable, trackable, trapped droplets in a fluidic system. Electrodes may be positioned underneath individual droplet traps in a microchannel to assay droplet contents and/or actuating droplets for the release of the droplets from corresponding traps. The disclosed technology may be used for detection of the results of solvent extraction processes including time-dependent quantification of metal ion concentration in the aqueous and organic phases, for wastewater treatment, heavy metal detection, pharmaceutical industry, and/or biotechnology, or for environmental monitoring of wastewater for toxic metal, monitoring of biological cell viability and proliferation, monitoring of extraction processes used in heavy metal mining, monitoring of extraction processes used in nuclear fuel processing, monitoring kinetics of enzyme processes, and/or assessing pharmacodynamics and drug efficacy.
1 . A method for determining a droplet's characteristic in a microfluidic device, the method comprising:
providing an electrical signal at one or more predetermined frequencies to a first electrode of the microfluidic device, the first electrode positioned within a microfluidic channel configured to allow fluid including one or more droplets to pass through the microfluidic channel;
sensing electrical signals from at least a second electrode of the microfluidic device, the second electrode positioned within the microfluidic channel and configured to produce an electrical signal upon making physical contact with, or upon being in close proximity to, a first droplet;
obtaining at least one impedance value based on the sensed electrical signals; and
determining at least one characteristic of the first droplet based on the at least one impedance value, wherein the microfluidic device comprises a plurality of traps and the first and the second electrodes are part of a first trap of the plurality of traps, each trap configured to arrest a movement of a droplet that makes physical contact with the trap, and wherein obtaining the at least one impedance value comprises actuating at least the first trap to determine the at least one impedance value of the trapped first droplet.
2 . The method of claim 1 , wherein the at least one characteristic includes one of a size or a content of the droplet.
3 . The method of claim 2 , comprising:
providing the electrical signal at a first frequency and determining one of the size or the content of the first droplet based on the at least one impedance value obtained at the first frequency, and
providing the electrical signal at a second frequency and determining the other of the size or the content of the first droplet based on the at least one impedance value obtained at the second frequency.
4 . The method of claim 1 , wherein the first and the second electrode are part of a first set of electrodes, and the method further comprises:
allowing the first droplet to travel through the microfluidic channel to reach a second set of electrodes, and
obtaining at least one additional impedance value from electrical signals sensed from the second set of electrodes, wherein:
determining the at least one characteristic of the first droplet includes determining an extraction associated with the first droplet based on impedance changes in the at least one additional impedance value obtained based on electrical signals sensed at the second set of electrodes compared to the at least one impedance value obtained based on electrical signals sensed at the first set of electrodes.
5 . The method of claim 4 , wherein:
the impedance changes caused by changes in a resistance value are indicative of extraction of ions,
the impedance changes caused by changes in a capacitance value are indicative of extraction of dipoles, and
the impedance changes caused by changes in an inductance value are indicative of extraction of metals.
6 . The method of claim 1 , wherein:
the first and the second electrode are part of a first set of electrodes,
the first set of electrodes further includes a third electrode, and
obtaining the at least one impedance value includes obtaining one or both of: an impedance value between the second electrode and the first electrode, or an impedance value between the third electrode and the second electrode.
7 . The method of claim 4 , wherein each of the first and the second set of electrodes include at least three electrodes.
8 . The method of claim 1 , wherein obtaining the at least one impedance value includes obtaining one or more of: a magnitude, a phase, a value of a real component or a value of an imaginary component of the at least one impedance value.
9 . The method of claim 1 , further comprising de-actuating at least the first trap to release the trapped droplet.
10 . The method of claim 1 , comprising, for each trap, providing the electrical signal at one or more frequencies, and allowing the trapped droplet to make physical contact with or become close in proximity to be electrically detected by at least two electrodes in each trap.
11 . The method of claim 1 , wherein the plurality of traps includes a plurality of rows and columns, and a plurality of droplets that flow through the microfluidic channel are intercepted by the plurality of traps.
12 . The method of claim 1 , comprising selectively actuating or de-actuating any one of the plurality of traps.
13 . The method of claim 1 , comprising using a demultiplexer to selectively control one or more of the plurality of traps.
14 . The method of claim 1 , comprising releasing the trapped droplet in response to an alternating current (AC) or direct current (DC) electric signal.
15 . The method of claim 1 , obtaining the at least one impedance value includes obtaining one or more of a magnitude, a phase, a value of a real component or a value of an imaginary component of the at least one impedance value.