IP Library › Granted Patent US 12,503,708
Granted Patent B2
US 12,503,708 · App. 17/819,296 · Granted Dec 23, 2025

Compositions and methods for enhancing resistance to northern leaf blight in maize

Inventors: Bailin Li (Johnston, IA); William Andrew Wilson (Noblesville, IN)
Assignee: CORTEVA AGRISCIENCE LLC
C12N15/8282A01H1/04A01H1/045A01H5/10A01H6/4684C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
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Quick Facts
Patent No.
US 12,503,708
App. No.
17/819,296
Granted
Dec 23, 2025
Kind
B2
Abstract

Provided herein are a maize plant comprising a heterologous DNA sequence that comprises an NLB18 sequence associated with resistance to northern leaf blight and a method of generating a maize plant comprising resistance to northern leaf blight.

Claims (22)

1 . A maize plant comprising a heterologous DNA sequence that comprises an NLB18 sequence associated with resistance to northern leaf blight, wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 95 or 97, and the heterologous DNA sequence is located on a chromosome other than chromosome 8.

2 . The maize plant of claim 1 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 95, and wherein the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:90: a C at position 61, a T at position 77, a G at position 82, a C at position 88, an A at position 113, a C at position 116, a G at position 117, a C at position 131, a T at position 143, an A at position 147, a T at position 156, a C at position 208, a G at position 237, an A at position 242, a T at position 243, a T at position 250, an A at position 267, a T at position 278, an A at position 289, a C at position 301, a T at position 302, a G at position 303, an A at position 354, a T at position 355, a G at position 360, a C at position 389, a C at position 399, an A at position 437, a T at position 445, a T at position 458, a C at position 491, an A at position 492, an A at position 493, an A at position 494, an A at position 495, an A at position 496, an A at position 497, an A at position 498, an A at position 499, an A at position 500, a T at position 557, and a CTG at position 588.

3 . The maize plant of claim 1 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 95, and wherein the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:91: a T at position 38, a G at position 46, a C at position 104, a G at position 107, a T at position 170, a T at position 173, an A at position 174, a G at position 234, an A at position 263, a G at position 277, an A at position 299, a C at position 368, a T at position 406, a C at position 421, a G at position 431, a G at position 435, an A at position 464, a G at position 492, an A at position 535.

4 . The maize plant of claim 1 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 97, and wherein the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:90: an A at position 45, an A at position 61, a T at position 77, an A at position 82, a C at position 88, an A at position 113, a C at position 116, a C at position 117, a T at position 131, a T at position 143, a T at position 156, a C at position 208, a G at position 237, an A at position 242, a T at position 243, a T at position 250, a G at position 267, a T at position 278, an A at position 289, a C at position 301, a T at position 302, a G at position 303, an A at position 354, an A at position 355, a C at position 360, a T at position 389, a C at position 399, an A at position 437, a T at position 445, an A at position 458, a C at position 491, an A at position 492, an A at position 493, an A at position 494, an A at position 495, an A at position 496, an A at position 497, an A at position 498, an A at position 499, a GTAACCA at position 519, a T at position 557, and a CTG at position 588.

5 . The maize plant of claim 1 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 97, and wherein the gene sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:91: a T at position 38, a T at position 46, a CGCTTG at position 94, a C at position 104, a T at position 107, a G at position 170, a T at position 173, a G at position 174, an A at position 234, an A at position 263, a G at position 277, an A at position 299, a C at position 368, a T at position 406, a C at position 421, a G at position 431, a G at position 435, an A at position 464, a G at position 492, an A at position 535.

6 . The maize plant of claim 1 , wherein the NLB18 sequence encodes SEQ ID NO: 95.

7 . The maize plant of claim 1 , wherein the NLB18 sequence encodes SEQ ID NO: 97.

8 . The maize plant of claim 1 , wherein the plant comprises the nucleic acid sequence of SEQ ID NO: 94.

9 . The maize plant of claim 1 , wherein the plant comprises the nucleic acid sequence of SEQ ID NO: 96.

10 . The maize plant of claim 1 , wherein the heterologous DNA sequence comprises a genetic stack comprising a plurality of genes.

11 . A method of generating a maize plant comprising resistance to northern leaf blight, the method comprising:

introducing a heterologous DNA sequence into one or more maize plant cells at a location other than chromosome 8, wherein the heterologous DNA sequence comprises an NLB18 sequence associated with resistance to northern leaf blight and the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 95 or 97; and

regenerating a plant from the maize plant cells, wherein the regenerated plant comprises the heterologous DNA sequence at a location other than chromosome 8.

12 . The method of claim 11 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 95, and the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:90: a C at position 61, a T at position 77, a G at position 82, a C at position 88, an A at position 113, a C at position 116, a G at position 117, a C at position 131, a T at position 143, an A at position 147, a T at position 156, a C at position 208, a G at position 237, an A at position 242, a T at position 243, a T at position 250, an A at position 267, a T at position 278, an A at position 289, a C at position 301, a T at position 302, a G at position 303, an A at position 354, a T at position 355, a G at position 360, a C at position 389, a C at position 399, an A at position 437, a T at position 445, a T at position 458, a C at position 491, an A at position 492, an A at position 493, an A at position 494, an A at position 495, an A at position 496, an A at position 497, an A at position 498, an A at position 499, an A at position 500, a T at position 557, and a CTG at position 588.

13 . The method of claim 11 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO:95, and the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:91: a T at position 38, a G at position 46, a C at position 104, a G at position 107, a T at position 170, a T at position 173, an A at position 174, a G at position 234, an A at position 263, a G at position 277, an A at position 299, a C at position 368, a T at position 406, a C at position 421, a G at position 431, a G at position 435, an A at position 464, a G at position 492, an A at position 535.

14 . The method of claim 11 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO:97, and the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:90: an A at position 45, an A at position 61, a T at position 77, an A at position 82, a C at position 88, an A at position 113, a C at position 116, a C at position 117, a T at position 131, a T at position 143, a T at position 156, a C at position 208, a G at position 237, an A at position 242, a T at position 243, a T at position 250, a G at position 267, a T at position 278, an A at position 289, a C at position 301, a T at position 302, a G at position 303, an A at position 354, an A at position 355, a C at position 360, a T at position 389, a C at position 399, an A at position 437, a T at position 445, an A at position 458, a C at position 491, an A at position 492, an A at position 493, an A at position 494, an A at position 495, an A at position 496, an A at position 497, an A at position 498, an A at position 499, a GTAACCA at position 519, a T at position 557, and a CTG at position 588.

15 . The method of claim 11 , wherein the NLB18 sequence encodes a polypeptide comprising at least 95% amino acid sequence identity to SEQ ID NO: 97, and the NLB18 sequence comprises one or more nucleotide polymorphisms corresponding to the following positions of SEQ ID NO:91: a T at position 38, a T at position 46, a CGCTTG at position 94, a C at position 104, a T at position 107, a G at position 170, a T at position 173, a G at position 174, an A at position 234, an A at position 263, a G at position 277, an A at position 299, a C at position 368, a T at position 406, a C at position 421, a G at position 431, a G at position 435, an A at position 464, a G at position 492, an A at position 535.

16 . The method of claim 11 , wherein the NLB18 sequence encodes SEQ ID NO: 95.

17 . The method of claim 11 , wherein the NLB18 sequence encodes SEQ ID NO: 97.

18 . The method of claim 11 , wherein the regenerated plant comprises the nucleic acid sequence of SEQ ID NO:94.

19 . The method of claim 11 , wherein the regenerated plant comprises the nucleic acid sequence of SEQ ID NO:96.

20 . The method of claim 11 , wherein the heterologous DNA sequence comprises a genetic stack comprising a plurality of genes.

Assignments (2)
NUNC PRO TUNC ASSIGNMENT Recorded Apr 16, 2025
From: E.I. DU PONT DE NEMOURS AND COMPANY
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 070865/0102 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 11, 2022
From: LI, BAILIN; WILSON, WILLIAM A.
To: E. I. DU PONT DE NEMOURS AND COMPANY
Reel/Frame 060789/0840 →
Continuity (5)
Continuation 14848390 · Sep 9, 2015
Continuation 14692850 · Apr 22, 2015
Continuation 13696153
Provisional Application 61358429 · Jun 25, 2010
Related Publication 20230203525A1 · Jun 29, 2023
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