Lipid vesicle compositions with penetration enhancing agents
The present application is related to a pharmaceutical composition a biphasic lipid vesicle comprising a lipid bilayer comprising vesicle forming lipids; an oil-in-water emulsion stabilized by one or more surfactants; one or more compounds; and one or more penetration enhancing agents. The one or more penetration enhancing agents include one or more non-ionic surfactants having a hydrophilic-lipophilic balance (HLB) of about 10 or less, alone or combination with one or more penetration enhancing agents selected from one or more of terpenes, alkaloids, salicylate derivatives, and polycationic surfactants and combinations thereof. The present application is also related to a pharmaceutical composition comprising a biphasic lipid vesicle comprising a lipid bilayer comprising vesicle forming lipids; an oil-in-water emulsion stabilized by one or more polycationic surfactants; and one or more compounds.
1. A biphasic lipid vesicle composition comprising:
a) one or more lipid vesicles comprising a lipid bilayer comprising vesicle forming lipids,
b) an oil-in-water emulsion entrapped in the biphasic lipid vesicles, wherein the oil-in-water emulsion comprises one or more components forming a non-covalent association with the lipid bilayer, wherein the oil-in-water emulsion comprises an aqueous continuous phase and a dispersed oil phase, and wherein the dispersed oil phase comprises droplets having an average diameter of less than 1 μm; and
c) one or more penetration enhancing agents entrapped in the lipid bilayer or the oil-in-water emulsion;
wherein the one or more penetration enhancing agents comprise one or more non-ionic surfactants having a hydrophilic-lipophilic balance (HLB) of about 10 or less comprising diethylene glycol monooleyl ether, sorbitan monopalmitate, or polyoxyethylene (4) dilaurate.
2. The composition of claim 1 , wherein the one or more penetration enhancing agents are entrapped in the oil-in-water emulsion.
3. The composition of claim 1 , wherein the one or more penetration enhancing agents are entrapped in the lipid bilayer and the oil-in-water emulsion.
4. The composition of claim 1 , wherein the lipid bilayer comprises about 10 wt %, about 9 wt %, about 8 wt %, about 7 wt %, about 6 wt %, about 5 wt %, about 4 wt %, about 3 wt %, about 2 wt %, about 1 wt %, about 0.5 wt % or about 0.1 wt % of the one or more penetration enhancing agents.
5. The composition of claim 1 , wherein the one or more penetration enhancing agents further comprise terpenes, alkaloids, salicylate derivatives, polycationic surfactants, or any combination thereof.
6. The composition of claim 5 , wherein the terpenes comprise eugenol, d-limonene, menthol, menthone, farnesol, neridol, camphor, nerol, thymol, or any combination thereof.
7. The composition of claim 5 , wherein the salicylate derivatives comprise ethyl salicylate, salicylic acid, acetylsalicylic acid, trolamine salicylate, or any combination thereof.
8. The composition of claim 5 , wherein the alkaloids comprise piperine, lobeline, caffeine, theobromine theophylline, nicotine, colchicine, N-methyl pyrrolidone, hygrine, capsaicin, berberine, sanguinarine, histamine, pilocarpine, or any combination thereof.
9. The composition of claim 5 , wherein the polycationic surfactants comprise one or more gemini cationic surfactants comprising a quaternary ammonium.
10. The composition of claim 5 , wherein the polycationic surfactants comprise polycationic amino acids.
11. The composition of claim 1 , wherein the oil-in-water emulsion of the biphasic lipid vesicles is stabilized by a second one or more surfactants comprising polyoxyethylene (10) cetyl ether and polysorbate 80.
12. The composition of claim 1 , wherein the vesicle forming lipids comprise one or more of phospholipids, glycolipids, or ceramides.
13. The composition of claim 1 , further comprising one or more compounds entrapped in the oil-in-water emulsion of the biphasic lipid vesicle, lipid bilayer of the biphasic lipid vesicle or both the oil-in-water emulsion and the lipid bilayer.
14. The composition of claim 13 , wherein the one or more compounds comprise a small molecule with a weight of up to 1000 Daltons, protein, peptide, carbohydrate, nucleic acid, vaccine antigen, plant extract, or any combination thereof.
15. The composition of claim 1 , wherein the biphasic lipid vesicle formulations further comprise one or more other lipid vesicle components comprising a fatty substance, penetration enhancer, surfactant, solvents, or any combination thereof.
16. The composition of claim 1 , wherein the one or more non-ionic surfactant comprises an oleyl ether.