Granted Patent B2

US 12,351,820 · App. 17/858,920 · Granted Jul 8, 2025

Inventors: Marianne J. Ellis (Caerdydd, GB); Julian Chaudhuri (Caerdydd, GB); Trevor Clive Dale (Caerdydd, GB)

Assignee: Molecular Devices (UK) Limited

C12N5/0081C12N5/0062C12N5/0679C12N2509/10C12N2513/00C12N2533/00C12N2533/50

Abstract

Claims (23)

1. A method for processing organoids, the method comprising:

culturing unprocessed organoids; and

disassociating the cultured unprocessed organoids to produce a cell suspension;

sieving the cell suspension through a cell strainer to obtain a sieved cell suspension containing cells of about 10 μm to about 1 mm in diameter; and

seeding cells of the sieved cell suspension into a bioreactor in a cell culture medium comprising an extracellular support matrix; and

recovering stage I organoids from the bioreactor;

wherein the bioreactor is a fed-plate bioreactor.

2. The method of claim 1 , wherein the fed-plate bioreactor is a flat-bed bioreactor.

3. The method of claim 1 , wherein the bioreactor is a perfusion bioreactor.

4. The method of claim 1 , wherein the fed-plate bioreactor comprises an arrangement of bioreactors that are fed in parallel or in series.

5. The method of claim 1 , wherein the cell culture medium comprises about 1% to about 99% v/v of the extracellular support matrix.

6. The method of claim 5 , wherein the cell culture medium comprises about 5% to about 85% v/v of the extracellular support matrix.

7. The method of claim 1 , wherein the extracellular support matrix is a solubilized basement membrane preparation.

8. The method of claim 1 , wherein the extracellular support matrix comprises laminin, entactin and collagen IV, or comprises laminin, entactin, collagen IV and heparin sulphate proteoglycan.

9. The method of claim 1 , wherein the cell strainer has a mesh size of about 30 μm to about 50 μm.

10. A method for processing organoids, the method comprising:

culturing unprocessed organoids;

disassociating the cultured unprocessed organoids to produce a cell suspension;

sieving the cell suspension through a cell strainer having a mesh size of about 30 μm to about 50 μm to obtain a sieved cell suspension; and

seeding cells of the sieved cell suspension into a bioreactor in a cell culture medium comprising an extracellular support matrix; and

recovering stage I organoids from the bioreactor.

11. The method according to claim 10 , wherein the bioreactor is a fed-plate bioreactor.

12. The method according to claim 10 , wherein the cell strainer has a mesh size of about 40 μm.

Assignments (2)

Priority Claims (1)

Continuity (2)

References Cited (29)