Well plate assembly with pressure release mechanism
A well plate assembly with an interior channel system in the well plate lid provides a more efficient and uniform distribution of fluid into a receptacle positioned below the well plate lid. The channel system in the lid allows air, or other fluid, to pass through with the use of a pump to each of a plurality of channels in the receptacle. Inlet and outlet valves in the lid prevent a gasket positioned between the lid and the receptacle from releasing contact with the receptacle due to high pressure experienced during the injection of fluid into the assembly. Specifically, pressure under a specified tolerance passes through the inlet valves, and if the pressure within the channel system exceeds the limit of the outlet valve, the outlet valve opens and allows air to escape the lid safely without disturbing the fluid flow into the channels below.
1. A receptacle lid comprising:
an inlet channel configured to receive fluid from a content source coupled to the receptacle lid;
a plurality of delivery valves, each delivery valve having an input configured to receive fluid from the inlet channel and an output configured to deliver fluid to a receptacle when the lid is connected to the receptacle;
a gasket, positioned between the receptacle lid and the receptacle, comprising a unitary sheet with a plurality of extrusions mapped to a size and shape of the receptacle; and
an interior channel system coupled to the inlet channel and configured to distribute the received fluid from the inlet channel to the inputs of the plurality of delivery valves, wherein the fluid is delivered to the receptacle through the delivery valves responsive to a pressure in the interior channel system exceeding a threshold.
2. The receptacle lid of claim 1 , wherein the receptacle is a well plate.
3. The receptacle lid of claim 1 , further comprising:
a plurality of lid clips extending downward from a bottom of the receptacle lid and configured to secure the receptacle lid to a bottom of the receptacle.
4. The receptacle lid of claim 1 , further comprising an outlet valve configured to open to allow release of fluid responsive to the pressure in the interior channel system exceeding a second threshold.
5. The receptacle lid of claim 4 , wherein the outlet valve is an umbrella valve.
6. The receptacle lid of claim 4 , wherein the outlet valve is positioned on a top surface of the receptacle lid.
7. The receptacle lid of claim 4 , wherein the inlet channel is positioned at a first end of the receptacle lid.
8. The receptacle lid of claim 7 , wherein the outlet valve is positioned at a second end of the receptacle lid, the second end opposite the first end.
9. The receptacle lid of claim 1 , wherein each delivery valve includes a sealing disc attached at a bottom of a valve stem, the sealing disc extending through an extrusion in the gasket and into the receptacle.
10. The receptacle lid of claim 1 , wherein the receptacle is a well plate and wherein the extrusions extend into each well of the well plate.
11. The receptacle lid of claim 1 , wherein a flow of fluid in the interior channel system is bidirectional.
12. The receptacle lid of claim 1 , wherein the threshold pressure is approximately three pounds per square inch (PSI).
13. The receptacle lid of claim 4 , wherein the second threshold pressure is approximately four pounds per square inch (PSI).
14. The receptacle lid of claim 1 , wherein the delivery valves are umbrella valves.
15. The receptacle lid of claim 14 , wherein a rate of fluid flow into a given receptacle varies based on a position of a corresponding umbrella valve.