Granted Patent B2

US 12,186,334 · App. 17/874,382 · Granted Jan 7, 2025

Inventors: Christopher Michael Kevin Springate (Richmond, CA); Ian Millet (Richmond, CA); Sailesh Haresh Daswani (Richmond, CA); Hesong Sun (Richmond, CA)

Assignee: ARC Medical Inc.

A61K31/737A61P41/00C08B37/0003C08B37/0063

Abstract

Claims (15)

1. A high-molecular-weight fucan wherein the high-molecular-weight fucan consists of a molecular weight distribution wherein at least about 92% w/w of the molecular weight distribution of the fucan is greater than 100 kDa when measured using an aqueous gel permeation chromatography set up consisting of:

one 300 mm analytical gel permeation chromatography column with a 7.8 mm inner diameter packed with hydroxylated polymethacrylate-based gel, having an effective molecular weight range of between about 50 kDa and about 5,000 kDa, one 300 mm analytical gel permeation chromatography column with a 7.8 mm inner diameter packed with hydroxylated polymethacrylate-based gel, having an effective molecular weight range of between about 1 kDa and about 6,000 kDa and one 40 mm guard column with a 6 mm inner diameter packed with hydroxylated polymethacrylate-based gel, the two analytical gel permeation chromatography columns and the one guard column contained in a column compartment at about 30° C.;

a refractive index detector at about 30° C.;

0.1M sodium nitrate mobile phase run at 0.6 mL/min; and

quantification against a peak molecular weight standard curve consisting of a first dextran standard with a peak molecular weight of about 2,200 kDa, a second dextran standard with a peak molecular weight of between about 720 kDa and about 760 kDa, a third dextran standard with a peak molecular weight between about 470 kDa and about 510 kDa, a fourth dextran standard with a peak molecular weight between about 370 kDa and about 410 kDa, a fifth dextran standard with a peak molecular weight between about 180 kDa and about 220 kDa, and a sixth dextran standard with a peak molecular weight between about 40 kDa and 55 kDa.

2. The high-molecular-weight fucan of claim 1 , comprising a weight average molecular weight between about 100 kDa and 10,000 kDa.

3. The high-molecular-weight fucan of claim 2 , wherein the weight average molecular weight is between about 370 kDa and 1900 kDa.

4. The high-molecular-weight fucan of claim 1 , comprising a number average molecular weight between about 50 kDa and 3,000 kDa.

5. The high-molecular-weight fucan of claim 1 , wherein at least 55% w/w of the distribution is greater than about 200 kDa.

6. The high-molecular-weight fucan of claim 1 , wherein at least 22% w/w of the distribution is greater than about 500 kDa.

7. A high-molecular-weight fucan wherein the high-molecular-weight fucan consists of a molecular weight distribution wherein between about 61% w/w and 80% w/w of the molecular weight distribution of the fucan is between about 200 kDa and 1600 kDa when measured using an aqueous gel permeation chromatography set up consisting of:

one 300 mm analytical gel permeation chromatography column with a 7.8 mm inner diameter packed with hydroxylated polymethacrylate-based gel, having an effective molecular weight range of between about 50 kDa and about 5,000 kDa, one 300 mm analytical gel permeation chromatography column with a 7.8 mm inner diameter packed with hydroxylated polymethacrylate-based gel, having an effective molecular weight range of between about 1 kDa and about 6,000 kDa and one 40 mm guard column with a 6 mm inner diameter packed with hydroxylated polymethacrylate-based gel, the two analytical gel permeation chromatography columns and the one guard column contained in a column compartment at about 30° C.;

a refractive index detector at about 30° C.;

0.1M sodium nitrate mobile phase run at 0.6 mL/min; and

quantification against a peak molecular weight standard curve consisting of a first dextran standard with a peak molecular weight of about 2,200 kDa, a second dextran standard with a peak molecular weight of between about 720 kDa and about 760 kDa, a third dextran standard with a peak molecular weight between about 470 kDa and about 510 kDa, a fourth dextran standard with a peak molecular weight between about 370 kDa and about 410 kDa, a fifth dextran standard with a peak molecular weight between about 180 kDa and about 220 kDa, and a sixth dextran standard with a peak molecular weight between about 40 kDa and 55 kDa.

Assignments (2)

Continuity (17)

References Cited (135)