Single-use disposable oxygen sensor
An electrochemical oxygen sensor includes a sensing surface having a working electrode and a reference electrode, a hydrophilic layer formed from an oxygen diffusion-limiting layer emulsion overlaying the working electrode and a hydrophobic membrane formed from a hydrophobic solution disposed over the hydrophilic layer. The hydrophilic layer contains an epoxy network and a hydrophilic polymer. The hydrophobic layer contains an acetate copolymer and a cross-linking agent that reacts with the liquid epoxy resin in the hydrophilic layer forming the epoxy network where the hydrophobic member is water vapor and oxygen permeable.
1. A multi-layer reagent matrix comprising:
a hydrophilic layer at least partially formed as an epoxy network containing a hydrophilic polymer; and
a hydrophobic layer disposed over the hydrophilic layer and comprising a cross-linking agent capable of reacting with a liquid epoxy resin of the hydrophilic layer in forming the epoxy network of the hydrophilic layer.
2. The multi-layer reagent matrix of claim 1 , wherein the hydrophilic polymer comprises a polyvinyl alcohol.
3. The multi-layer reagent matrix of claim 1 , wherein the hydrophobic layer comprises an acetate copolymer.
4. The multi-layer reagent matrix of claim 3 , wherein the acetate copolymer comprises an ethylene vinyl acetate.
5. The multi-layer reagent matrix of claim 4 , wherein 50% of the ethylene vinyl acetate is polymerized in an ethylene vinyl polymer backbone.
6. The multi-layer reagent matrix of claim 1 , wherein the hydrophobic layer is water vapor and oxygen permeable.
7. A method for making a multi-layer reagent matrix, the method comprising:
forming a hydrophilic emulsion by combining a predefined amount of liquid epoxy resin, a predefined amount of a hydrophilic polymer, a predefined amount of a surfactant, and a predefined amount of distilled water; and
forming a membrane solution for depositing onto the hydrophilic layer by combining a predefined amount of an acetate copolymer, a predefined amount of an epoxy curing agent, and a predefined amount of pentaerythritol tetrakis 3-mercaptopropionate.
8. The method of claim 7 , wherein forming the hydrophilic emulsion further comprises:
combining a predefined amount of an anti-foaming agent with the liquid epoxy resin, hydrophilic polymer, surfactant, and distilled water.
9. The method of claim 7 , wherein the predefined amount of liquid epoxy resin is 1.6 grams, the predefined amount of hydrophilic polymer is 1.4 grams, and the predefined amount of distilled water is 1 milliliter.
10. The method of claim 7 , wherein the predefined amount of the acetate copolymer is 50 wt %, the predefined amount of the epoxy curing agent is 3 wt %, and the predefined amount of pentaerythritol tetrakis 3-mercaptopropionate is 1 wt %.
11. The method of claim 7 , wherein the hydrophilic polymer comprises a polyvinyl alcohol.
12. The method of claim 7 , further comprising:
drying the hydrophilic emulsion to form a hydrophilic layer.
13. The method of claim 12 , further comprising:
depositing the membrane solution onto the hydrophilic layer.
14. The method of claim 13 , further comprising:
drying the membrane solution to form a hydrophobic layer on the hydrophilic layer.
15. The method of claim 12 , further comprising:
reacting the liquid epoxy resin and the epoxy curing agent to at least partially form the hydrophilic layer as an epoxy network containing the hydrophilic polymer.
16. A method of forming an electrochemical oxygen sensor comprising the multi-layer reagent matrix of claim 7 , the method comprising:
providing a sensor body having a base layer with at least two independent conductive paths and an insulating and reagent holding layer disposed onto the base layer, wherein the insulating and reagent holding layer has at least two reagent holding openings, wherein one of the at least two reagent holding openings exposes a portion of one of the at least two independent conductive paths and the other of the at least two reagent holding openings exposes a portion of another of the at least two independent conductive paths;
disposing the hydrophilic emulsion into one of the at least two reagent holding openings and drying the hydrophilic emulsion to form a hydrophilic layer; and
disposing the membrane solution over the hydrophilic layer and drying the membrane solution to form a hydrophobic layer;
wherein the one of the at least two reagent holding openings containing the hydrophilic layer and the hydrophobic layer forms a working electrode of the electrochemical oxygen sensor.
17. An electrochemical oxygen sensor comprising:
a sensing surface having a working electrode and a reference electrode;
a hydrophilic layer overlaying the working electrode, wherein the hydrophilic layer is at least partially formed as an epoxy network containing a hydrophilic polymer; and
a hydrophobic layer disposed over the hydrophilic layer, the hydrophobic layer containing a cross-linking agent capable of reacting with a liquid epoxy resin of the hydrophilic layer in forming the epoxy network of the hydrophilic layer.
18. The electrochemical oxygen sensor of claim 17 , wherein the sensing surface has a base layer with at least two independent conductive paths and an insulating and reagent holding layer disposed onto the base layer, wherein the insulating and reagent holding layer has at least two reagent holding openings, wherein one of the at least two reagent holding openings exposes a portion of the at least two independent conductive paths and the other of the at least two reagent holding openings exposes a portion of another of the at least two independent conductive paths.
19. The electrochemical oxygen sensor of claim 17 , wherein the hydrophilic polymer comprises a polyvinyl alcohol.