In vitro fertilization system and components associated therewith
Described herein are devices, systems, and methods to aid in the manipulation of cells. The devices, methods, and systems disclosed herein can be applied towards, for example, automation of the in vitro fertilization process.
1 . A biochip comprising:
(a) a first layer comprising a plurality of reservoirs;
(b) a second layer comprising:
(i) a plurality of channels imprinted in the second layer, wherein the channels are in fluidic communication with the reservoirs;
(ii) a plurality of valves configured to control liquid flow within the plurality of channels; and
(iii) at least one well imprinted in the second layer fluidically connected to the plurality of reservoirs by the plurality of channels,
wherein the well comprises:
(I) an open upper end;
(II) a closed lower end;
(III) a perimeter body connecting the closed lower end and the open upper end;
(IV) an inlet; and
(V) an outlet
wherein:
the well is dimensioned to receive a cell or a group of cells;
the size of the inlet in a first dimension is larger than a diameter of the cell or group of cells;
the size of the inlet in a second dimension is smaller than the diameter of the cell or group of cells;
the size of the outlet in a first dimension is larger than the diameter of the cell or group of cells; and
the size of the outlet in a second dimension is smaller than the diameter of the cell or group of cells;
the well is placed between one inner valve placed before the inlet and one outer valve placed after the outlet;
wherein the second dimension of the inlet and the second dimension of the outlet become larger before joining one of the plurality of channels; and
(c) a housing encasing the first layer and the second layer.
2 . The biochip of claim 1 , wherein the second layer further comprises:
(iv) an upper film that seals the channels from the top; and
(v) a lower film that seals the channels from the bottom.
3 . The biochip of claim 1 , further comprising:
(d) a plurality of orifices, wherein the plurality of orifices is above the plurality of reservoirs;
(e) a pressure source; and
(f) a sealing layer in between and in contact with the plurality of reservoirs and the pressure source;
wherein the sealing layer provides a pneumatic seal between the pressure source and the plurality of reservoirs.
4 . The biochip of claim 1 , further comprising:
(d) a storage pot imprinted in the second layer; and
(e) an air permeable medium covering the storage pot.
5 . The biochip of claim 1 , wherein the well further comprises a suction port.
6 . The biochip of claim 1 , wherein each well has two adjacent metal electrodes acting as capacitive sensors.
7 . The biochip of claim 1 , wherein the closed lower end of the well is level with at least one of the plurality of channels.
8 . A method comprising:
(a) depositing a cell or group of cells into a well of a biochip according to claim 1 ,
(b) performing a liquid exchange, wherein the liquid exchange comprises:
(I) flowing a first liquid into the well through an inner valve and through the inlet; and
(II) flowing a second liquid out of the well through the outlet and through an outer valve;
wherein the liquid exchange is performed to promote fertilization or embryo development of the group of cells.
9 . The method of claim 8 , further comprising, after (b), performing a second liquid exchange, wherein the second liquid exchange comprises:
(III) flowing a third liquid into the well through the inner valve and through the inlet; and
(IV) flowing the first liquid out of the well through the outlet and through the outer valve.
10 . The method of claim 8 further comprising:
(a) generating a conditioned media by culturing a cell or group of cells in a media within a well,
(b) connecting a negative pressure port to a storage pot, wherein:
the storage pot is covered by an air permeable medium; and
the storage pot is fluidically connected to the outlet through the outer valve;
(c) drawing gas pressure through the negative pressure port out of the storage pot causing the conditioned media to flow through the outlet and through the outer valve into the storage pot such that the storage pot fills with the conditioned media and the conditioned media approaches the air permeable medium; and
(d) ceasing to draw the conditioned media into the storage pot when the conditioned media contacts the air permeable medium.
11 . A system comprising:
(a) a biochip of claim 1 ; and
(b) an actuation cradle, wherein the biochip fits into the actuation cradle.
12 . The system of claim 11 , wherein the plurality of valves is configured to be controlled by the actuation cradle.
13 . The system of claim 11 , wherein the actuation cradle comprises a plurality of activation pins engaging with the plurality of valves.
14 . The system of claim 11 , wherein the actuation cradle further comprises one or more temperature, capacitive, or flow sensors.
15 . The system of claim 11 , wherein the cradle comprises one or more pumps.