IP Library › Granted Patent US 12,186,279
Granted Patent B2
US 12,186,279 · App. 17/903,286 · Granted Jan 7, 2025

Sustained release cowpea mosaic virus or virus-like particle therapeutic constructs for the treatment of cancer

Inventors: Nicole F. Steinmetz (San Diego, CA); Frank Alexander Veliz (Cleveland, OH); Brylee David B. Tiu (Cleveland, OH)
Assignee: CASE WESTERN RESERVE UNIVERSITY
A61K47/6901A61K39/0011A61K39/385A61K39/39A61P35/00A61K2039/5258A61K2039/585A61K2039/622A61K2039/645B82Y5/00
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Quick Facts
Patent No.
US 12,186,279
App. No.
17/903,286
Granted
Jan 7, 2025
Kind
B2
Abstract

A nanoparticle construct includes a plurality of plant virus or virus-like particles electrostatically coupled to a plurality of nanoparticles having a different surface charge than the plant virus or virus-like particles. The nanoparticle construct upon delivery to a subject can provide a sustained release of the plant virus or virus-like particles and/or nanoparticles to a cell or tissue of the subject.

Claims (12)

1. A nanoparticle construct for in situ delivery of plant virus or plant virus-like particles to a cell or tissue of interest of a subject, the nanoparticle construct comprising:

a plurality of plant virus or plant virus-like particles, wherein the plant virus or plant virus-like particle is a rod-shaped virus particle, a plant picornavirus, or a filamentous plant virus or plant virus-like particle; and

a plurality of dendrimers electrostatically coupled to the plant virus or plant virus-like particles, the nanoparticle construct upon in situ delivery to a subject providing a sustained release of the plant virus or plant virus-like particles and/or dendrimers to the cell or tissue.

2. The nanoparticle construct of claim 1 , having a hydrodynamic radius of about 100 nm to about two microns.

3. The nanoparticle construct of claim 1 , upon administration to a subject disassembling in a sustained manner at physiological salt concentrations.

4. The nanoparticle construct of claim 1 , wherein the plant virus or virus-like particle is of the Secoviridae family or Alphafexiviridae family.

5. The nanoparticle construct of claim 1 , wherein the plant virus or virus-like particle is a cowpea mosaic virus-like particle or potato virus X virus-like particle.

6. The nanoparticle construct of claim 1 , wherein the rod-shaped virus is a tobacco mosaic virus.

7. The nanoparticle construct of claim 1 , wherein the dendrimer is a G3-G10 dendrimer.

8. The nanoparticle construct of claim 1 , comprising alternating electrostatically coupled layers of the plant virus or virus-like particles and the dendrimers.

9. The nanoparticle construct of claim 1 , wherein the plant virus or virus-like particle is loaded with or bonded to a cargo molecule.

10. The nanoparticle construct of claim 9 , wherein the cargo molecule is an anticancer agent.

Assignments (2)
CONFIRMATORY LICENSE Recorded Feb 19, 2025
From: CASE WESTERN RESERVE UNIVERSITY
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 070253/0409 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 25, 2024
From: STEINMETZ, NICOLE F.; VELIZ, FRANK ALEXANDER; TIU, BRYLEE DAVID B.
To: CASE WESTERN RESERVE UNIVERSITY
Reel/Frame 069042/0541 →
Continuity (3)
Continuation 16319765
Provisional Application 62364949 · Jul 21, 2016
Related Publication 20230076403A1 · Mar 9, 2023
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