Seaweed cultivation method and system
The present invention relates to a system and method for cultivating an seaweed, preferably Sarcothalia crispata , including releasing spores from seaweed reproductive tissue, inoculating a substrate with spores, germinating the spores and growing seaweed from the germinated spores. The invention also relates to a harvested batch of seaweed having a lambda carrageenan content of at least about 80 wt. % based on mass of all carrageenans and wherein the mass of seaweed is at least 100 kg, more preferably at least 500 kg and most preferably at least 1000 kg.
1. A method of cultivating carageenophytic seaweed, the method comprising:
a. providing a reproductive tissue of the seaweed;
b. releasing spores from the reproductive tissue by:
(i) contacting the reproductive tissue with a non-saline aqueous solution;
(ii) at least partially dehydrating the reproductive tissue;
(iii) contacting said at least partially dehydrated tissue with a first saline solution to produce a spore-containing saline; and
(iv) optionally filtering the spore-containing saline;
c. adding the spore-containing saline to a container containing a second saline solution and a substrate and allowing the spores to settle on the substrate, wherein said substrate is at least partially immersed in said second saline solution;
d. germinating spores on the substrate; and
e. growing the seaweed from the germinated spores.
2. The method of claim 1 wherein step b.(i) comprises contacting the seaweed reproductive tissue with a non-saline aqueous solution for at most 1 hour.
3. The method of claim 1 , wherein the substrate is a net.
4. The method of claim 1 , wherein the substrate, prior to use, is washed with a non-saline aqueous solution, is sterilized with boiling or near-boiling water, or combinations thereof.
5. The method of claim 1 , wherein the spore-containing saline produced in step b. has a spore concentration of 60-65 million spores per litre.
6. The method of claim 1 , wherein the second saline solution in the container of step c. has a spore concentration of 4.8 million to 5.2 million spores per litre.
7. The method of claim 1 , wherein the number of spores germinated on the substrate at step d. is at least 150 per linear centimetre substrate.
8. The method of claim 1 , wherein the germinating step d. includes the steps of washing the substrate, washing the vessel, or combinations thereof.
9. The method of claim 1 , wherein the seaweed is Sarcothalia crispata.
10. The method of claim 9 wherein the seaweed reproductive tissue is obtained from the cystocarpic phase of the seaweed.
11. The method of claim 1 , further comprising transferring to the sea either the germinated spores of step d. or partially grown seaweed produced by step e. and growing the seaweed in the sea.
12. The method of claim 11 further comprising harvesting the seaweed from the sea.
13. The method of claim 1 , wherein the reproductive tissue of the seaweed comprises fronds containing spores.